| 刘敏燕，苏程瑜，张润桃，梁卓玲，黄月权，张晓明，欧阳乐军.基本培养基对尾巨桉愈伤组织诱导及再生的影响[J].广东农业科学,2013,40(13):50-52 |
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基本培养基对尾巨桉愈伤组织诱导及再生的影响 |
| Effects of basal mediums on callus induction and plant regeneration of Eucalyptus urophylla ×伊E. grandis |
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| DOI： |
| 中文关键词: 尾巨桉 基本培养基 愈伤组织诱导 再生 |
| 英文关键词: Eucalyptus urophylla ×E. grandis basal mediums callus induction regeneration |
| 基金项目: |
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| 中文摘要: |
| 为建立尾巨桉高效再生体系，研究基本培养基对尾巨桉再生的影响，以尾巨桉优良无性系无菌苗茎段为外植体，探讨MS、SDM和SPM 3种基本培养对尾巨桉愈伤组织诱导、不定芽分化以及增殖的影响。结果表明，在添加了2 mg/L PBU和0.05 mg/LIAA的MS培养基上，茎段外植体5 d后愈伤组织诱导率达96.3%以上；将愈伤组织接种在添加1 mg/L 6-BA和0.05 mg/L NAA的MS培养基上，诱芽率达91.8%，不同基本培养基对不定芽的增殖及伸长的影响差异达到极显著水平。 |
| 英文摘要: |
| The callus induction and shoot regeneration were studied systematically in elite clone of Eucalyptus urophylla ×E. grandis by comparing the basal mediums. The results showed that sterile clones of seedling stem segments were used as explants and cultured in a MS medium supplemented with the synthetic growth regulator 2 mg/L PBU and 0.05 mg/L IAA. After cultivation for 5 d, 96.3% of explants formed callus. Callus were transferred to MS medium containing 1 mg/L 6-BA and 0.05 mg/L NAA to induce bud formation, and 91.8% of the callus induced by PBU exhibited adventitious bud formation. Effect of basic mediums on the adventitious shoot proliferation and development from hypocotyls were greatly significant differences. |
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