| 陈晓燕，王军辉，姚玉飞，杜逸群，孙汉巨，张建波.竹笋多糖复合酶法辅助提取及抗氧化活性研究[J].广东农业科学,2014,41(4):113-117 |
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竹笋多糖复合酶法辅助提取及抗氧化活性研究 |
| Optimal process of extraction with combined-enzyme method andantioxidant activity of polysaccharide from Bamboo shoots |
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| DOI： |
| 中文关键词: 复合酶法 竹笋水溶性多糖 提取 抗氧化活性 |
| 英文关键词: complex enzymes method bamboo shoots water-soluble polysaccharide extraction antioxidant activity |
| 基金项目:安徽省国际科技合作计划项目（12030603020）；江
南大学食品胶体与生物技术教育部重点实验室开放基金（JDSJ20
12-04） |
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| 中文摘要: |
| 探讨了竹笋多糖复合酶法辅助提取及醇沉工艺，并对其体外抗氧化活性进行研究，为其工业化生产
提供参考。结果表明，复合酶最佳添加量为每0.3 g 样品添加纤维素酶、果胶酶、木瓜蛋白酶分别为360、1 080、7
200 U；最佳提取条件为院提取温度60益，提取时间2.2 h，料液比1颐35；多糖最佳醇沉条件为80%乙醇20益醇沉4
h；抗氧化活性结果表明竹笋多糖具有较高的体外抗氧化活性，对DPPH 自由基和ABTS 自由基的清除率分别达到
88.1%和86.5%。 |
| 英文摘要: |
| The compound enzymatic extraction and alcohol precipitation process of bamboo polysaccharides was
investigated, antioxidant activity in vitro was determined as well. The experimental results indicated that the optimal
contents of cellulase, pectinase and papain were 360, 1 080, 7 200 U per 0.3 g sample. The optimal extraction conditions
of bamboo polysaccharide were extraction time 2.2 h, extraction temperature 60益and solid to liquid ratio of 1 颐35.
Subsequently, the optimum conditions of alcohol precipitation process on bamboo polysaccharides were volume percent of
ethanol 80%, temperature 20益, and alcohol precipitation time 4 h. Furthermore, the crude polysaccharide showed good
antioxidant activities, and its scavenging activities of DPPH free radical and ABTS free radical approached 88.1% and
86.5%, respectively. |
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