| 张帅，刘飞，朱华伟，等.Plackett-Burman设计和响应面法优化单宁酶固态发酵培养基[J].广东农业科学,2015,42(12):91-96 |
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Plackett-Burman设计和响应面法优化单宁酶固态发酵培养基 |
| Optimization of solid state fermentation medium for tannase by Plackett-Burman design and response surface methodology |
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| DOI： |
| 中文关键词: 单宁酶 发酵培养基 Plackett-Burman 设计 响应面法 中心组合设计 |
| 英文关键词: tannase fermentation medium Plackett-Burman (PB) design response surface methodology (RSM) central composite design (CCD |
| 基金项目: |
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| 摘要点击次数: 1416 |
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| 中文摘要: |
| 通过优化单宁酶固态发酵培养基组成，以期最大程度提高产酶活力。首先采用Plackett-Burman 设
计对固态发酵培养基组分进行重要性筛选，并确定可显著影响单宁酶活力的3 种组分：麸皮、水及单宁酸。通
过最速上升试验接近最大响应区域后，再经中心组合设计响应面试验建立二次回归模型，发酵培养基最优组
成确定为：麸皮9.23 g，水0.78 mL，单宁酸0.76 g，NH4NO3 1.20 g，MnCl2 0.024 g，NaCl 0.004 g，MgSO4窑7H2O
0.004 g，KH2PO4 0.004 g。在此条件下发酵单宁酶，酶活力达462.72 U/mL，与模型预测值467.57 U/mL 非常接近。
结果表明，利用Plackett-Burman 设计和响应面法优化单宁酶固态发酵培养基非常有效。 |
| 英文摘要: |
| Solid state fermentation medium for tannase was optimized to improve tannase activity. Plackett-
Burman design was firstly used to screen important components of the medium, and three components with significant
effect on tannase activity were bran, water and tannic acid. After steepest ascent experiment approaching the optimal
region of these factors, response surface experiment of center composite design (CCD) was applied to establish the
quadratic regression model for tannase activity. The optimal medium compositions were obtained as follows: 9.23 g
bran, 0.78 mL H2O, 0.76 g tannic acid, 1.20 g NH4NO3, 0.024 g MnCl2, 0.004 g NaCl, 0.004 g MgSO4 7H2O and
0.004 g KH2PO4. The activity value of tannase fermented under the above conditions was 462.72 U/mL, which was
nearly in agreed with the predictive value 467.57 U/mL. The results indicated that it was very effective for
optimization of solid state fermentation medium for tannase by using Plackett-Burman design and response surface
methodology. |
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