| 严婉荣，赵志祥，肖 敏，陈 圆，符尚娇，陈绵才.海南省辣椒、番茄病毒病病原
DAS-ELISA 定性检测[J].广东农业科学,2015,42(18):68-72 |
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海南省辣椒、番茄病毒病病原
DAS-ELISA 定性检测 |
| DAS-ELISA detection of pepper and tomatoviruses in Hainan province |
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| DOI： |
| 中文关键词: 辣椒 番茄 病毒病 双抗体夹心酶联免疫吸附测定法 |
| 英文关键词: pepper tomato virus DAS-ELISA |
| 基金项目:：海南省自然科学基金（314157）；国家公
益性行业（农业）科研专项（201303028）；海南省农科
院科技创新基金（琼农院［2014］32 号） |
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| 中文摘要: |
| 辣椒和番茄是海南省重要的经济作物，但病毒病的发生导致严重减产。2013-2014 年，在全省范
围内采集了253 份辣椒病毒病样品和289 番茄病毒病样品，采用DAS-ELISA 对7 种主要病毒及病毒的相对含
量进行了测定。结果显示，辣椒病毒病的总体检出率为59.68%，CMV 的检出率最高，为41.11%，存在7 种复
合侵染类型，复合侵染率为30.83%，优势毒源为CMV 和TMV。番茄病毒病的总体检出率为44.64%，TYLCV
的检出率最高，为22.84%，存在10 种复合侵染类型，复合侵染率为15.57%，优势毒源为TYLCV 和CMV。样
品相对含量测定显示，在辣椒中CMV 的相对含量最高，BBWV 的含量最低；在番茄中TYLCV 的相对含量最
高，BBWV 的含量最低；辣椒样品中除CMV 含量高于番茄外，其他病毒的含量均低于番茄。 |
| 英文摘要: |
| Peppers and tomatoes are important economical agricultural crops，several viruses cause yield losses
of pepper and tomato. In 2013-2014，253 pepper and 289 tomato virus disease samples were collected in Hainan
province，7 main viruses and the relative content of virus were measured by double antibody sandwich enzyme-linked
immunosorbent assay（DAS-ELISA）. The results showed that the overall detection rate of pepper virus disease was
59.68% and CMV was the highest（41.11%）. There were 7 compound infection types and infection rate was 30.83%，
CMV and TMV were the advantage virus sources of pepper. The overall detection rate of tomato virus disease was 44.64%
and TYLCV was the highest（22.84%）. There were 10 compound infection types and infection rate was 15.57%，
TYLCV and CMV were the advantage virus sources of tomato. Determination of the relative content of pepper samples
showed that CMV was the highest and BBWV was the lowest. Determination of the relative content of tomato samples
showed that TYLCV was the highest and BBWV was the lowest. The content of CMV was higher in pepper samples than
that in tomato samples，but the content of other viruses were lower. |
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