| 洪鹏志，张 鹏，韩道财，杨 萍.罗非鱼肉蛋白酶解液及其超滤液
的抗氧化活性研究[J].广东农业科学,2015,42(20):102-106 |
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罗非鱼肉蛋白酶解液及其超滤液
的抗氧化活性研究 |
| Study on antioxidant activity of Tilapiahydrolysate and its ultrafiltrate |
| |
| DOI： |
| 中文关键词: 酶解液 超滤液 自由基 抗氧化活性 氨基酸含量 |
| 英文关键词: hydrolysate ultrafiltrate free radical scavenging antioxidant activity amino acid content |
| 基金项目:广东海洋大学创新强校工程项目
（GDOU2013050313，GDOU2014050203）；省部产学研合
作专项（2013B090800021） |
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| 中文摘要: |
| 通过研究罗非鱼肉蛋白酶解液及其5 ku 超滤液的体外抗氧化活性，为罗非鱼深加工及高值化利
用提供理论基础。主要采用分光光度法分别检测了酶解液和超滤液的还原力，对·OH、O2
-·、DPPH·自由
基的清除能力以及在亚油酸体系中抗氧化能力并对其抗氧化活性的差异性进行分析讨论。结果发现，当浓度
为1.25~2.5 mg/mL 时，超滤液的还原力、在亚油酸体系中抗氧化能力低于酶解液，当浓度为2.5~10.0 mg/mL
时，超滤液活性强于酶解液；以IC50 计算，超滤液对自由基清除能力均优于酶解液；超滤液中亮氨酸、苯丙氨
酸、异亮氨酸、组氨酸和酪氨酸的含量均高于酶解液，其分子量小于3 ku 与1 ku 组分的比例也高于酶解液。
罗非鱼肉蛋白酶解液经5 ku 超滤处理后，其体外抗氧化活性增强。 |
| 英文摘要: |
| The vitro antioxidant activities of tilapia hydrolysate and its 5 ku ultrafiltrate were studied aimed at
offering basic theory for tilapia processing and high-value utilization. The capacities on reducing power，·OH，O2
-·，
DPPH·free radical scavenging and its antioxidation in linoleic acid of hydrolyzate and ultrafiltrate were investigated
by spectrophotometry，as well as an in-depth analysis on its antioxidant activity variability was carried out. The results
showed that when the concentration was 1.25-2.5 mg/mL，the reducing power and antioxidation in linoleic acid of
ultrafiltrate was lower than those of hydrolyzate，while the concentration was 2.5-10 mg/mL，the result was opposite.
And the free radical scavenging of ultrafiltrate was better than that of hydrolyzate with half inhibitory concentration
（IC50）. The contents of Leu，Phe，Ile，His and Tyr in ultrafiltrate were higher than those in hydrolysate，in addition，
the components which were lower than 3 ku or 1 ku，in ultrafiltrate were higher than those in hydrolysate. The results
indicated that compared with tilapia hydrolysate，the antioxidant activity of 5 ku ultrafiltrate obviously increased |
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