| 周国华1，2.水稻冷诱导蛋白激酶基因CIPK07g
的克隆与表达分析[J].广东农业科学,2015,42(21):150-155 |
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水稻冷诱导蛋白激酶基因CIPK07g
的克隆与表达分析 |
| Cloning and expression analysis of cold induced proteinkinase gene CIPK07g in rice（Oryza sativa L.） |
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| DOI： |
| 中文关键词: 水稻 冷刺激 蛋白激酶基因 克隆 表达 |
| 英文关键词: rice cold treatment protein kinase gene clone expression |
| 基金项目:国家自然科学基金（31200219）；云南省
教育厅基金（2012Y371 |
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| 摘要点击次数: 1224 |
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| 中文摘要: |
| CIPK 是一类Ca2+ 依赖的蛋白激酶超基因家族。对水稻中该基因家族成员之一CIPK07g 进行克
隆，并对其编码蛋白的信号肽、跨膜结构、结构功能域、时空表达特性和低温处理的表达特征进行分析。结果
表明，CIPK07g 基因cDNA 全长1 891 bp，包含279 bp 5′UTR 及259 bp 3′UTR，编码450 个氨基酸组成的多肽
链。该基因蛋白N 端无信号肽，含跨膜结构域，为亲水蛋白，具有CIPK 基因家族特有的保守结构域，在叶片
中表达较高，低温刺激后24 h 表达达到高峰。 |
| 英文摘要: |
| The gene CIPK07g in rice was cloned，which was one of the members of Ca2+-dependent protein kinase
superfamily，and it’s signal peptide，transmemberane domain，conserved domain，expression profile in different
organisms and low temperature treatment were analyzed. The results showed that，the cDNA full length of CIPK07g gene
was 1 891 bp，including 279 bp 5′UTR and 259 bp 3′UTR，which encoded a polypeptide with 450 amino acids. There
was a characteristic conserved domain of CIPK gene family in CIPK07g protein，and the protein was a hydrophilicity
protein which included a transmemberane domain but had no signal peptide in N-terminal analyzed by bioinformatic
tools. The CIPK07g gene was highly expressed in leaf after 24 h cold treatment. |
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