| 王凯强，彭 晴，乔 宇，丁 慧，徐小轻，张宇微，魏晨阳，石 波.大豆细胞悬浮培养及其大豆异黄酮和抗毒素诱导积累研究进展[J].广东农业科学,2017,44(3):30-38 |
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大豆细胞悬浮培养及其大豆异黄酮和抗毒素诱导积累研究进展 |
| Advances in suspension-cultured methods of soybeancell and accumulation on soybean isoflavonesand glyceollins from cell exposed to elicitor |
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| DOI：10.16768/j.issn.1004-874X.2017.03.005 |
| 中文关键词: 大豆 细胞悬浮培养 诱导子 诱导 大豆异黄酮 抗毒素 |
| 英文关键词: soybean cell suspension-cultured elicitor elicition soybean isoflavones glyceollins |
| 基金项目:国家自然科学基金（31171628） |
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| 摘要点击次数: 3077 |
| 全文下载次数: 1737 |
| 中文摘要: |
| 大豆细胞悬浮培养是将大豆细胞及细胞团培养于液体培养基中的方法，应用于大豆多种研究
领域。从不同的外植体包括子叶、子叶节、下胚轴、幼胚、茎培养大豆细胞的方法，外源诱导物及前体对细
胞积累大豆异黄酮以及诱导物对细胞合成和积累大豆抗毒素诱导效果进行综述。大豆异黄酮和抗毒素是大
豆产生的两类具有生物活性的次生代谢物。在此基础上，指出诱导中存在的问题及今后的研究方向，旨在
通过增加诱导子的选择而促进大豆细胞合成更高水平抗毒素及异黄酮提供一些思路。 |
| 英文摘要: |
| Suspension-cultured cell technique which developed soybean cell and aggregates in liquid medium，
has been extensively applied in numerous fields. The paper summarized the methods for suspension-cultured soybean
cell employing different explants，including cotyledon，cotyledon node，hypocotyl，immature embryo and stem，
inducible effect of exogenous elicitors and precursor treatment on accumulation of isoflavones and influence of elicitors
exposure to soybean cell on synthesis and accumulation of glyceollins. Soybean isoflavones and glyceollins are two
types of bioactive secondary metabolites produced from soybean. On the basis，the present problems were illustrated
and prospective development directions were also recommended for the propose of providing ideas concerning higher
level of isoflavones and glyceollins accumulation produced by cell in response to other optional elicitors. |
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