吴 萍,李青苗,夏燕莉,郭俊霞,王晓宇,肖 特,方清茂,张 美.甘油对超低温保存白芷种子活力的影响[J].广东农业科学,2017,44(3):47-51 |
Effects of glycerol on seed vigor of Angelica dahuricaunder cryopreservation |
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DOI:10.16768/j.issn.1004-874X.2017.03.007 |
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Abstract: |
The effects of glycerol treatment on seed vigor of Angelica dahurica under cryopreservation was
investigated. The same amount of seeds were mixed with glycerol in different volume ratio and placed in the freezing
tube. Then,the mixture was thawed at room temperature for 24 hours after storing one week under cryopreservation.
The germination ability,conductivity,peroxidase( POD) activity and soluble protein content of seed were
determined. The results showed that:glycerol had some positive effects on improving seed vigor,and the best effect
was the treatment with 10% glycerol,in which the seed germination potentiality,germination index,vigor index were
significantly enhanced. In the process of seed cryopreservation,glycerol could reduce seed conductivity,increase
soluble protein content and POD activity of seeds. The correlation coefficient between conductivity,soluble protein
content or POD activity and seed vigor index was -0.93,0.79 or 0.71,which can be used as measurable indicators of A.
dahurica seed vigor. |
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