| 盛 慧，杜伟玲.黄瓜Ca2+ 家族序列特征及其表达特性分析[J].广东农业科学,2017,44(3):61-66 |
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黄瓜Ca2+ 家族序列特征及其表达特性分析 |
| Analysis on sequence characteristics and expressionof Ca2+ family of cucumber |
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| DOI：10.16768/j.issn.1004-874X.2017.03.009 |
| 中文关键词: 黄瓜 Ca2+ 家族 组织特异性 信号传导 |
| 英文关键词: cucumber Ca2+ family tissue specificity signal transduction |
| 基金项目:哈尔滨市科技攻关计划项目（2006HE6BE052） |
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| 摘要点击次数: 2276 |
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| 中文摘要: |
| 通过黄瓜基因组数据库，采用生物信息学方法获得15 个黄瓜Ca2+ 家族序列，并对其染色体
位置、基因组位置、氨基酸长度、转录起始位点和Pfam 匹配结构域数进行预测；利用RT-PCR 方法对所
有搜索到的Ca2+ 家族基因进行组织特异性分析；对黄瓜植株进行低温处理，分析黄瓜Ca2+ 基因家族的表
达情况。结果显示：15 个基因中除Csa021245 未定位以外，其余均匀分布在1～6 号染色体上，一致性为
47.67%；Csa001688、Csa009857 和Csa009360 等3 个基因的5′区出现CpG 岛结构；组织表达特异性分析
结果显示，Csa001688、Csa006826、Csa010172 和Csa016434 在叶中表达量最高，Csa003758、Csa010172 和
Csa018949 在根中表达量最高；进化树分析结果显示，只有Csa010172 和Csa009360 与拟南芥AtACA2 亲缘
关系最近，这些基因可能在Ca2+ 家族信号传导过程中起着主要作用；实时定量表达分析表明，Ca2+ 基因家
族参与黄瓜对低温胁迫的应答。 |
| 英文摘要: |
| Fifteen sequences of Ca2+ family in cucumber were indentified from Cucumber Genome Database
using bioinformatics methods，and the chromosomal location，genomic position，amino acid length，transcription
initiation site and Pfam matching domain were predicted. At the same time，sequence similarity and phylogenetic
tree were analyzed. Cucumber plants were exposed to low temperature，and the expression of Ca2+ gene family was
analyzed. The results showed that 5 sequences distributed on the first chromosome，3 sequences distributed on the
second chromosome，2 sequences distributed on the fourth chromosome，1 sequence was not located，the remaining
sequences evenly distributed on other chromosomes. With RT-PCR technique，the expressions of Csa001688，
Csa006826，Csa010172 and Csa016434 in leaves were higher， the expressions of Csa003758，Csa010172 and
Csa018949 in roots were higher. Phylogenetic tree analysis showed that，only Csa010172 and Csa009360 were
close with Arabidopsis AtACA2，these genes may play important roles in Ca2+ family signal transduction. Real - time
quantitative expression analysis showed that Ca2+ gene family was involved response to low temperature stress in
cucumber. |
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