| 张锦东1，王小玉2，游淑珠2，赵国锋3，余以刚1.菠萝蜜种子中总黄酮的提取工艺及其抗氧化性研究[J].广东农业科学,2017,44(12):136-143 |
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菠萝蜜种子中总黄酮的提取工艺及其抗氧化性研究 |
| Total flavonoids extraction and antioxidant activity from jackfruit seed |
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| DOI：10.16768/j.issn.1004-874X.2017.12.021 |
| 中文关键词: 菠萝蜜种子 正交实验设计 总黄酮 抗氧化性 自由基 |
| 英文关键词: jackfruit seed orthogonal experimental design total flavonoids antioxidant activity free radicals |
| 基金项目:广东省科技计划项目（2016B090918103）；珠海进出口公共技术服务平台产学研协同创新计划项目
（IETP201601006） |
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| 摘要点击次数: 2292 |
| 全文下载次数: 582 |
| 中文摘要: |
| 采用四因素三水平正交试验设计优化了菠萝蜜种子中总黄酮的提取工艺。结果表明，在水浴条
件下，4 个因素对菠萝蜜种子粉的总黄酮提取率的影响大小为：乙醇浓度＞料液比＞提取温度＞提取时间。
其中，乙醇浓度对总黄酮提取率影响极显著，料液比、提取温度、提取时间对总黄酮提取率影响显著。水浴
条件下的最佳提取工艺为：70% 乙醇，60℃水浴提取50 min，料液比1∶20 g/mL，菠萝蜜种子中总黄酮提取
率达到3.30%。采用DPPH 法和水杨酸法测定菠萝蜜种子中总黄酮粗提液的抗氧化活性，结果表明，总黄酮
粗提液浓度在0.6 mg/mL 时两种测定方法中自由基的清除率均超过90%，具有较强的抗氧化活性。实际生
产中，菠萝蜜种子可作为抗氧化剂的原料来源。 |
| 英文摘要: |
| The extraction technology of total flavonoids from jackfruit seed powder was studied by four factors
three levels orthogonal experiment. The results showed that the effect order of four factors on the extraction rate of
total flavonoids from seed powder of jackfruit was：ethanol concentration > liquid ratio > extraction temperature >
extraction time. The ethanol concentration had a significant effect on the total flavonoids extraction rate（ P<0.01），
the ratio of material to liquid，the extraction temperature and extraction time had significant effects on the total
flavonoids extraction rate（ P<0.05）. When the ethanol concentration was 70%，the extraction temperature was
60℃，the extraction time was 50 min，the ratio of material to liquid was 1∶20，the optimum extraction process
under the condition of water bath was reached. The extraction rate of total flavonoids from jackfruit seed powder under
the best extraction process conditions reached 3.30%. The antioxidant activity of crude flavonoids from jackfruit seed
powder was determined by DPPH method and salicylic acid method. The results showed that the scavenging rate of free radicals was more than 90% at 0.6 mg/mL，indicating that the flavonoids from jackfruit seed powder had strong
antioxidant activity and had good application prospect. In actual production，the seed of jackfruit can be used as the
source of antioxidants. |
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