| 景凤霞，李云琪，唐灵杰，李小锦，王宇辉，张秀敏.不同营养因子对土壤细菌可培养物种多样性的影响[J].广东农业科学,2018,45(8):65-72 |
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不同营养因子对土壤细菌可培养物种多样性的影响 |
| Effects of different nutritional factors on culturable species diversity of soil bacteria |
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| DOI：10.16768/j.issn.1004-874X.2018.08.010 |
| 中文关键词: 营养因子 难培养微生物 系统发育分析 细菌分离 多样性 潜在新种 |
| 英文关键词: nutrition factors refractory microorganism phylogenetic analysis bacterial separation diversity potential new species |
| 基金项目:国家自然科学基金（31270053）；河北省自然科学基金（C2014201141） |
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| 摘要点击次数: 1915 |
| 全文下载次数: 706 |
| 中文摘要: |
| 通过在改良的VL55 培养基中添加6 种不同的营养因子，利用大量稀释法对采自河北、青海
和云南的3 份土壤样品进行细菌分离，并对所分离菌株进行16S rRNA 基因序列系统发育分析，比较利用
不同营养因子分离的可培养细菌物种多样性。结果表明，添加营养因子分离得到的细菌物种多样性明显高
于对照；土样1 对照分离得到5 个属，添加6 种营养因子的处理共分离得到22 个属；土样2 对照分离得
到2 个属，添加6 种营养因子的处理共分离得到17 个属；土样3 对照分离得到6 个属，添加6 种营养因
子的处理分离得到15 个属。添加不同营养因子的培养基组合分离得到各自独特的细菌类群。其中，从青
海土样中分离得到的菌株HBUM200206 与运动东菌（Dongia mobilis）LM22T 的亲缘关系最近，序列相似
性为94.58%，可能是红螺菌科（Rhodospirillaceae）的一个潜在新属。此外，有12 株菌与亲缘关系最近的
模式菌株的16S rRNA 基因序列相似性均小于98.65%，可能为潜在的新种。 |
| 英文摘要: |
| By adding six different nutrient factors to the modified VL55 medium, a large number of dilutions
were used to isolate bacteria from three soil samples collected from three regions of Hebei, Qinghai, and Yunnan.
The phylogenetic analysis of the 16S rRNA gene sequences was performed on the strains isolated using six different
nutrient factors to compare the species diversity of culturable bacteria. The results showed that the bacterial species
diversity obtained from the addition of nutritional factors was significantly higher than that of the control; 5 genera
were isolated from the soil sample 1 control, 22 genera were isolated from the experimental group with the addition of
six nutritional factors; soil sample 2 control was isolated two genera, 17 genera were isolated from the experimental
group; 6 genera were isolated from the soil sample 3 control, and 15 genera were isolated from the experimental
group. The combination of culture media with different nutrient factors was added to isolate their unique bacterial
populations. Among them, the strain HBUM200206 isolated from the soil sample of Qinghai Province had the closest
genetic relationship with Dongia mobilis LM22T, and the 16S rRNA gene sequence similarity was 94.58%, which
may be a potential new genera of Rhodospirillaceae. In addition, the 16S rRNA gene sequence similarity of the 12
strains and the closest relatives of the type strains were all less than 98.65%, which may be potential new species. |
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