| 刘开平1，韦玉梅1，熊 杰1，孙燕飞1，雷勇辉2.东亚飞蝗和刺腿食蚜蝇内生酵母菌多样性分析[J].广东农业科学,2018,45(9):90-95 |
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东亚飞蝗和刺腿食蚜蝇内生酵母菌多样性分析 |
| Analysis of diversity of endobiotic yeasts isolated from Locusta migratoria manilensis and Ischiodonscutellaris Fabricius |
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| DOI：10.16768/j.issn.1004-874X.2018.09.015 |
| 中文关键词: 东亚飞蝗 刺腿食蚜蝇 内生酵母菌 高通量测序 物种多样性 |
| 英文关键词: Locusta migratoria manilensis Ischiodon scutellaris Fabricius endobiotic yeast high-throughput
sequencing species diversity |
| 基金项目:国家自然科学基金（31860003）；新疆特色植物基因资源研究与利用创新团队（2014CC005） |
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| 中文摘要: |
| 为探究东亚飞蝗和刺腿食蚜蝇内生酵母菌的菌群多样性和探索这两种在农业生态系统中具
有不同功能的昆虫内生酵母菌的多样性差异，通过Illumina MiSeq 高通量测序技术对2 个样本内生酵母
菌26SrDNA 进行测序，利用 Mothur 软件计算东亚飞蝗和食蚜蝇体内酵母菌的操作分类单元（operational
taxonomic unit , OTU）并进行丰富度Alpha 多样性分析，利用SPSS 软件进行多样性差异显著性分析，共产
生906 个OTU，东亚飞蝗中含647 个OTU，而食蚜蝇为677 个OUT，共有OUT 418 个；酵母菌共鉴定出2
门19 属。结果表明刺腿食蚜蝇内生酵母菌多样性略高于东亚飞蝗。东亚飞蝗体内发现了特有的掷孢酵母
属（Sporobolomyces）和拟威尔嗜杀酵母（Cyberlindnera）生防酵母资源。新疆东亚飞蝗和刺腿食蚜蝇的内
生酵母菌丰富多样，其生存的微环境具有特殊性。通过对其进一步研究可能为害虫防治和特殊环境下的酵
母菌资源利用提供新思路。 |
| 英文摘要: |
| To study the structural diversity of the endobiotic yeast isolated from Locusta migratoria manilensis
and Ischiodon scutellaris Fabricius and compare diversity of endobiotic yeasts isolated from insdects with differenet
ecologic functions inagriculture ecosystem, we sequenced 26SrDNA of endobiotic yeast from two samples by Illumina
Miseq high-throughput sequencing method, and classified operational taxonomic unit and analysed the Alpha diversity
by Mouthor software. And then，we analysed the signifiance of diversity difference by SPSS, a total of 906 OTU were
detected, 647 OTU from Locusta migratoria migratoria and 677 OTU from Ischiodon scutellaris Fabricius. among
which 418 OTU are shared in two samples. Yeasts were identified and classified into 2 plylums and 19 genus in the
two samples. The biodiversity of endobiotic yeas in Locusta migratoria migratoria is little more than that in Ischiodon
scutellaris Fabricius. Meanwhile, we identified biological control yeasts belongs to Sporobolomyces and Cyberlindnera
only in Locusta migratoria migratoria The endobiotic yeasts isolated from Locusta migratoria migratoria and
Ischiodon scutellaris Fabricius in Xinjiang are abundant and various. The micro-environment they existed is special.
It could provide new ideas for pest control and yeasts resoueses utilization in special environment. |
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