| 孙 辉，程东美.放线菌 A50 的鉴定及其对植物病原菌的抑制作用…[J].广东农业科学,2018,45(12):82-87 |
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放线菌 A50 的鉴定及其对植物病原菌的抑制作用… |
| Identification and antifungal activity against plant pathogens of actinomycetes strain A50 |
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| DOI：10.16768/j.issn.1004-874X.2018.12.014 |
| 中文关键词: 放线菌 分子鉴定 理化特性 植物病原菌 抑菌活性 |
| 英文关键词: actinomycetes molecular identification physiology and biochemistry characteristics plant
pathogen antagonism |
| 基金项目:广东省现代农业花卉产业技术体系创新团队病虫害防控岗位专家建设项目（2017LM1131） |
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| 中文摘要: |
| 为筛选新的具有抑菌活性的放线菌，采用稀释平板法从深圳赤坳水库保护林地芒箕土壤中分
离筛选得到 1 株放线菌 A50，并对 A50 进行初步鉴定以及抑菌活性测定。根据形态学特征、生理生化特性
和基于 16S rDNA 序列及系统发育分析等方面的研究结果 , 菌株 A50 鉴定为 Streptomyceyes nodosus。采用对
峙平板法测定 A50 的抑菌活性，结果表明，放线菌菌株 A50 对 5 种供试植物病原真菌菌丝生长均有不同
程度的抑制作用，其中以对玉米小斑病（Bipolaris maydis）的抑菌效果最好 , 抑菌带宽度达 1.5 cm。A50 发
酵液原液浸泡处理花生白绢病菌核，处理后 72 h 抑制率达 68%，稀释 20 倍后仍有 9% 的抑制率。 放线菌
A50 是一株具有良好开发应用前景的生防菌株。 |
| 英文摘要: |
| This study aimed to obtain actomycetes with high activity against plant pathogens from soil. Soil
samples were collected from woodlands of Lake Chi’ao in Shenzhen. Actinomycetes strain A50 was isolated using
serial dilution and plating method on Gause's Synthetic Agar Medium. Based on morphological, physiological and
biochemical characteristics, as well as the analysis of 16S rDNA, the strain A50 was was identified as Streptomyceyes
nodosus. The antifungal activity of A50 was studied by flat confrontation. The result of mycelium growth rate showed
that A50 had a broad spectrum inhibitory activity to all the tested 5 plant pathogenic fungi. Among these, activity
against Bipolaris maydis was the highest with the width of inhibitory zone above1.5cm. The fermentation solution of
A50 displayed 68% inhibition activity to sclerotium treated after 72h. A50 had big potential and wide prospect for
controlling plant pathogens. |
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