文章摘要
徐  匆 1 ,罗华建 1 ,李艳芳 1 ,黄  皓 1 ,梁卫驱 1 ,胡  珊 1 ,胡楚维 1 ,罗鸿斌 2.环介导等温扩增技术的应用及研究进展[J].广东农业科学,2019,46(4):116-123
环介导等温扩增技术的应用及研究进展
Application and Progress of Loop-mediated Isothermal Amplification Technology
  
DOI:10.16768/j.issn.1004-874X.2019.04.017
中文关键词: 环介导等温扩增(LAMP)  实时 LAMP  微流控 LAMP  横向流 LAMP  电化学 LAMP
英文关键词: loop-mediated isothermal amplification (LAMP)  real-time LAMP  micro-fluidic LAMP  lateral flow detect (LFD) LAMP  electrochemical LAMP
基金项目:广东省科技计划项目(2017A020208002)
作者单位
徐  匆 1 ,罗华建 1 ,李艳芳 1 ,黄  皓 1 ,梁卫驱 1 ,胡  珊 1 ,胡楚维 1 ,罗鸿斌 2 1. 东莞市农业科学研究中心广东 东莞 523086 2. 东莞市理工学院生态环境工程技术研发中心广东 东莞 523808 
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中文摘要:
      环介导等温扩增(LAMP)技术自 2000 年被发明以来以其快速、灵敏、低耗等特点,引起众多研 究者的关注。该技术在人、动物、植物、环境等相关微生物的检测、监控等领域得到了广泛研究与应用,其检 测灵敏度普遍高于传统 PCR 。但是传统 LAMP 技术对于产物检测存在耗时、易造成污染等问题,以及在野外或 医疗点检测时存在判读不便的弊端。就 LAMP 技术与其他多种产物检测技术结合后的方法如目视 LAMP、实时 LAMP、微流控 LAMP、横向流 LAMP 及电化学 LAMP 进行了总结、举例及讨论。目视 LAMP 具有费用低、适合 多环境下使用的特点;实时 LAMP、微流控及电化学 LAMP法能够有效提高样品检测的准确度,且可减少检测时间; 横向流 LAMP 具有可操作性强、便于判读等特点。根据目前 LAMP 技术的研究进展及各技术优劣势的分析,预 测 LAMP 技术未来发展的方向和重点可能为研发手持便携式检测仪。
英文摘要:
      Loop-mediated isothermal amplification (LAMP) technology has attracted many researchers’attention for its fast, sensitive and low-consumption characteristics since it was developed in 2000. This method has been widely studied and applied in the detection and monitoring of microorganisms related to human, animals, plants and environment. Its detection sensitivity is generally higher than that of traditional PCR. However, the traditional LAMP is time-consuming and easy to cause pollution in the detection of products, and has disadvantages of inconvenient interpretation in the field or point of care. This paper summarized, illustrated and discussed the methods of LAMP technology combining with other product detection technologies. Visual LAMP has the characteristics of low cost and being suitable for multi-environment. Real-time, micro-fluidic and electrochemical LAMP can effectively improve the accuracy of sample detection and reduce detection time. Lateral flow detect LAMP has the characteristics of strong operability and easy interpretation. According to the current research progress of LAMP technologies and the analysis of their advantages and disadvantages, it is predicted that the future development direction and focus of LAMP technology may be the development of hand-held portable detectors.
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