文章摘要
郑晓雅 1 ,殷伟伟 1 ,张 松 1 ,兰 瑛 2.不同发酵条件对柱状田头菇菌丝产胞外多糖的影响[J].广东农业科学,2019,46(5):25-32
不同发酵条件对柱状田头菇菌丝产胞外多糖的影响
Effects of Different Fermentation Conditions on Extracellular Polysaccharides Produced by Mycelia of Agrocybe aegerita
  
DOI:10.16768/j.issn.1004-874X.2019.05.004
中文关键词: 液体发酵  条件优化  多糖产量  碳源  氮源  苯酚 - 硫酸法
英文关键词: liquid fermentation  condition optimization  polysaccharide yield  carbon source  nitrogen source  phenolsulfuric acid method
基金项目::广东省科技计划项目(2016A010105019);广州市科技计划项目(201707010138);深圳市科技计划项目(CYZZ20160526154742735)
作者单位
郑晓雅 1 ,殷伟伟 1 ,张 松 1 ,兰 瑛 2 1. 华南师范大学生命科学学院广东 广州 510631 2. 深圳市汇尚科科技有限公司广东 深圳 518055 
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中文摘要:
      【目的】为了提高胞外多糖的产量,优化柱状田头菇液体发酵适宜的培养基和培养条件。【方法】 以胞外多糖产量为主要测定指标,通过对培养基的碳源、氮源种类及其浓度、起始 pH、摇床转速、培养时间等 发酵条件进行单因素试验,以获得柱状田头菇液体深层发酵的优化条件,提高柱状田头菇菌丝产胞外多糖的产量, 其中胞外多糖产量通过苯酚 - 硫酸法测得。【结果】柱状田头菇液体发酵产胞外多糖的优化条件为:采用可溶 性淀粉作为碳源,浓度控制在 20~40 g/L;采用 L- 谷氨酰胺作为氮源,浓度控制在 2 g/L;起始 pH 控制在 7.0, 转速控制在 180 r/min,并在 26 ℃下培养 12 d。【结论】在此发酵条件下,柱状田头菇液体发酵的胞外多糖产量 可达到 1.08 g/L,其中多糖含量为 71.73%、蛋白质含量为 8.62%,可为柱状田头菇多糖的工业化生产和应用提供 理论依据。
英文摘要:
      【Objective】 In order to improve the production of extracellular polysaccharides of Agrocybe aegerita, the suitable medium and related culture conditions for liquid fermentation were optimized. 【Method】 Based on the yield of extracellular polysaccharides, some fermentation conditions such as carbon source, nitrogen source and concentration, initial pH, shaking speed and culture time were explored by single factor test. The optimized conditions of submerged fermentation increased the yield of extracellular polysaccharide produced by the mycelium of A. aegerita, and the yield of extracellular polysaccharide was measured by the phenol-sulfuric acid method. 【Result】 The optimum conditions for the production of extracellular polysaccharides by liquid fermentation of A. aegerita were as follows: soluble starch was used as carbon source and the concentration was controlled at 20-40 g/L; L-glutamine was used as nitrogen source and the concentration was controlled at 2 g/L; the initial pH was controlled at 7.0, the shaking speed was controlled at 180 r/min, and extracellular polysaccharides were cultured at 26℃ for 12 d. 【Conclusion】Under the above-mentioned fermentation conditions, the yield of extracellular polysaccharide was 1.08 g/L, in which the content of polysaccharide was 71.73% and the content of protein was 8.62%. This provided an theoretical basis for the industrial production and application of A. aegerita polysaccharides.
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