文章摘要
刘丽娜 1,2,傅曼琴 2,徐玉娟 2,余元善 2,温 靖 2,吴继军 2.玉米芯膳食纤维的复合酶法改性工艺优化[J].广东农业科学,2019,46(8):138-145
玉米芯膳食纤维的复合酶法改性工艺优化
Optimization of Mixed Enzymatic ModificationProcess of Corncob Dietary Fiber
  
DOI:10.16768/j.issn.1004-874X.2019.08.019
中文关键词: 玉米芯  膳食纤维  纤维素酶  木聚糖酶  改性
英文关键词: corncob  dietary fiber  cellulase  xylanase  modification
基金项目:国家2019-05-12公益性行业(农业)科研专项(201503142-03);广东省科技计划项目(2016B020203003,2017A070702007)
作者单位
刘丽娜 1,2,傅曼琴 2,徐玉娟 2,余元善 2,温 靖 2,吴继军 2 1. 广东海洋大学食品科技学院广东 湛江 524088 2. 广东省农业科学院蚕业与农产品加工研究所 / 农业部功能食品重点实验室 / 广东省农产品加工重点实验室广东 广州 510610 
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中文摘要:
      【目的】建立酶法提取玉米芯膳食纤维方法,优化复合酶法改性玉米芯不溶性膳食纤维(IDF)制 备可溶性膳食纤维(SDF)工艺。【方法】以玉米芯为原料,通过单因素试验优化碱性蛋白酶、α- 淀粉酶和糖 化酶预处理提取玉米芯粗膳食纤维(TDF)条件,结合正交试验优化复合酶(纤维素酶和木聚糖酶)法改性 IDF 制备 SDF 工艺。【结果】生物酶法提取玉米芯 TDF 条件:料液比 1 ∶ 10、pH 9.0、1.4% 碱性蛋白酶 50 ℃酶解 60 min;pH 6.5、0.3% 的 α- 淀粉酶和糖化酶(1 ∶ 1)、60 ℃水解 60 min,IDF 得率为 69.35%。复合酶法改 性 IDF 最佳工艺为:pH 5.0、温度 50 ℃、纤维素酶 1.2%、木聚糖酶 1.2%、酶解时间为 6 h、料液比为 1 ∶ 10, SDF 得率可达 22.16%。处理后的 SDF 持水力为 6.55 g/g,膨胀性为 6.69 mL/g,持油力为 4.65 g/g,分别比改性前 提高 40.26%、48.67%、74.16%。【结论】复合酶法改性玉米芯 IDF 制备 SDF 得率较单一纤维素酶和单一木聚糖 酶处理的 SDF 得率高,且显著提高产物 SDF 的持水力、持油力和膨胀性。
英文摘要:
      【Objective】The study was to establish a method for enzymatic extraction of corncob dietary fiber and to optimize the preparation of soluble dietary fiber (SDF) by mixed enzymatic modification of corncob insoluble dietary fibe (IDF). 【Method】 With the corncob as raw material, the conditions of alkaline protease, α-amylase and saccharification enzyme pretreatment for extraction of corncob total dietary fiber (TDF) were optimized by single factor experiment. With the combination of single factor and orthogonal design experiments, the process of SDF preparation from IDF by mixed cellulase and xylanase modification was optimized. 【Result】 The conditions of bio-enzymatic extraction of corncob TDF were as follows: solid-liquid ratio of 1∶10, pH 9.0 and 1.4% alkaline protease with enzymatic hydrolysis at 50℃ for 60 min; pH 6.5, 0.3% α-amylase and glucoamylase (1 ∶ 1)with hydrolysis at 60℃ for 60 min, and IDF yield was 69.35%. The optimum process for the modification of IDF by mixed enzymatic method was as follows: pH 5.0, temperature 50 ℃ , cellulase 1.2%, xylanase 1.2%, with enzymatic hydrolysis for 6 h and the solid-liquid ratio of 1 ∶ 10. The SDF yield could reach 22.16%. The water holding capacity, expansibility, and oil binding capacity of the obtained SDF were 6.55 g/g, 6.69 mL/g, and 4.65 g/ g, respectively, which were 40.26%, 48.67% and 74.16% higher than those before modification, respectively. 【Conclusion】 The yield of SDF prepared by mixed enzyme modification from corncob IDF was higher than that of single cellulase or single xylanase, and the water holding capacity, oil binding capacity, and expansibility of SDF product were significantly improved as well.
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