| 陈兵先，张 琪，戴彰言，高家东，张文虎，吴柔贤，宋松泉，刘 军.水稻种子 TTC 染色及萌发特性研究[J].广东农业科学,2021,48(7):1-8 |
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水稻种子 TTC 染色及萌发特性研究 |
| Study on TTC Staining and Germination Characteristics of Rice Seeds |
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| DOI：10.16768/j.issn.1004-874X.2021.07.001 |
| 中文关键词: 水稻 TTC 染色 种子生活力 种子萌发 淀粉酶 |
| 英文关键词: rice TTC staining seed viability seed germination amylase |
| 基金项目:广东省自然科学基金面上项目（2020A1515011535）；广州市基础与应用基础研究项目（202002030403）；广东省农作物种质资源保存与利用重点实验室项目（2020B121201008） |
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| 中文摘要: |
| 【目的】开展水稻种子 TTC 染色试验条件及最佳组合筛选，探讨水稻种子萌发过程中主要生理指
标的变化规律，对于完善水稻种子质量检测具有重要指导意义。【方法】应用单因素试验和正交试验设计比较
种子吸水时间、TTC 浓度、染色温度和染色时间 4 个因素对染色效果的影响，并筛选最佳的 TTC 染色试验组合；
应用分光光度法测定不同萌发条件下水稻种胚内的淀粉酶活性和可溶性糖含量。【结果】被 TTC 染色的水稻种
胚分为 5 种情况：整个胚染色较深，整个胚染色较浅，胚根被染色，胚芽被染色，盾片被染色。4 个因素对水稻
种子生活力的影响依次为：染色时间＞染色温度＞ TTC 浓度＞吸水时间；水稻种子染色的最佳条件为：染色时
间 2 h，染色温度 30℃，TTC 浓度 1%，吸水时间 3 h。在不同萌发条件下，水稻种子中 α- 淀粉酶、β- 淀粉活
性和可溶性糖含量的变化与种子萌发率的变化趋势保持一致。【结论】TTC 染色最佳组合下，水稻种子染色率
能够准确地反映种子的生活力和萌发能力。淀粉酶活性和可溶性糖含量或可作为预测和评价水稻种子活力的重
要生理指标。 |
| 英文摘要: |
| 【Objective】It has important guiding significance for perfecting rice seed quality inspection to carry
out TTC staining experiment, select the best combination and explore the changes in the main physiological indexes of
rice seeds.【Method】Single factor analysis and orthogonal experimental design were used to compare the influence of
seed imbibition time, TTC concentration, staining temperature and staining time, on the staining effect, and to screen
the best TCC staining combination. Spectrophotometry was used to determine the amylase activity and soluble sugar
content in rice embryos under different germination conditions.【Result】Rice embryos stained by TTC are divided
into five situations: The entire embryo was stained darkly; The entire embryo was stained lightly; The coleorhiza was
stained; The coleoptile was stained; The scutellum was stained. The influence of the four factors on the viability of rice seeds is as follows: staining time ＞ staining temperature ＞ TTC concentration ＞ imbibition time; The best condition for
TTC staining of rice seeds is as follows: staining time 2 h, staining temperature 30℃ , TTC concentration 1%, imbibition
time 3 h. Under different germination conditions, the changes of α-amylase, β-amylase activity and soluble sugars in
rice seeds were consistent with the changes in seed germination rate. 【Conclusion】Under the best combination of TTC
staining, the staining rate of rice seeds could accurately reflect the viability and germination ability of the seeds. Amylase
activity and soluble sugar content may be used as important physiological indicators for predicting and evaluating rice
seed vigor. |
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