| 史绍琪 1，陈金甜 2，林毓娥 1，谢国源 3，黄美华 4，王 敏 1.白皮和江屯青皮佛手瓜嫩梢的代谢组比较分析[J].广东农业科学,2023,50(2):42-48 |
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白皮和江屯青皮佛手瓜嫩梢的代谢组比较分析 |
| Metabolomics Comparative Analysis on Tender Tips of White Sechium edule and Jiangtun Cyan Sechium edule |
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| DOI：10.16768/j.issn.1004-874X.2023.02.005 |
| 中文关键词: 佛手瓜 代谢组比较 差异代谢物 KEGG 育种 |
| 英文关键词: Sechium edule metabolomics differential metabolite KEGG breeding |
| 基金项目:广东省农业科学院科技创新战略专项（高水平农科院建设）（R2021PY-QF008）；广东省农业科学院农业优势产业学科团队建设项目（202103TD，202114TD）；广东省对外科技合作平台项目（2020A0505020006） |
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| 中文摘要: |
| 【目的】挖掘影响龙须菜品质和口感的代谢物质，对白皮和江屯青皮两种佛手瓜嫩梢进行代谢组分析。【方法】采用超高效液相色谱串联质谱（UPLC-MS/MS）技术，对白皮和江屯青皮两种佛手瓜嫩梢进行代谢组学检测，采用 MetaboAnalyst 5.0、SIMCA-P 和 Origin 等软件对代谢组数据进行分析。【结果】两种佛手瓜嫩梢间共筛选到 135 种差异代谢物，其中下调代谢物 82 个、上调 53 个；差异代谢物聚类法分析显示，芥子醛在白皮佛手瓜嫩梢中含量高，其次为松柏醇；在江屯青皮佛手瓜嫩梢中，检测差异代谢物溶血磷脂酰乙醇胺最高，其次为溶血磷脂酰胆碱；KEGG 结果显示差异代谢物显著富集于 5 条代谢途径，分别为嘌呤代谢、亚油酸新陈代谢、类黄酮生物合成、次生代谢产物合成及 α- 亚麻酸代谢途径。【结论】江屯青皮佛手瓜嫩梢中的脂质、黄酮类、生物碱以及核苷酸类等物质含量高，特别是溶血磷脂酰乙醇胺、日当药黄素、腺嘌呤核苷三磷酸和阿魏酰亚精胺等。研究结果为解析江屯青皮龙须菜代谢组学的营养和药用选育价值及优质品种提供理论基础。 |
| 英文摘要: |
| 【Objective】In order to find out the substances that affect the quality and taste of Chaylte Vine, the tender tips of white and Jiangtun cyan Sechium edule were used for metabonomic analysis.【Method】Metabolomic detection was carried out for tender tips of the two Sechium edule by using ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS), and MetaboAnalyst 5.0, SIMCA-P and Origin software were used to analyze metabolome data.【Result】The results showed that there were significant differences in metabolites among tender tips of the two Sechium edule. A total of 135 differential metabolites were screened, including 82 down-regulated metabolites and 53 up-regulated metabolites. The cluster analysis of differential metabolites showed that the content of myrosinaldehyde in white Sechium edule was high, followed by coniferol. The lysophosphatidyl ethanolamine was the highest differential metabolite in Jiangtun cyan Sechium edule, followed by lysophosphatidylcholine. The result of KEGG shows that differential metabolites enriched in 5 major metabolic pathways, including purine, linoleic acid, flavonoid biosynthesis, synthesis of secondary metabolites and α-linolenic acid metabolism.【Conclusion】The lipid, flavonoid, alkaloids and nucleotides substances in the tender tips of Jiangtun cyan Sechium edule are high, especially lysophosphatidyl ethanolamine, daidzein, adenosine triphosphate and ferulic spermidine etc. The research results elucidated the nutritional and medicinal basis of the metabolomics of Jiangtun cyan
Sechium edule, and provided a theoretical basis for the selection and breeding of high-quality varieties of Chaylte Vine. |
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