| 郭 义，李鹏燕，李敦松.荔枝蒂蛀虫不同地理种群遗传多样性初步研究[J].广东农业科学,2024,51(9):111-128 |
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荔枝蒂蛀虫不同地理种群遗传多样性初步研究 |
| Preliminary Study on Genetic Diversity of Different Geographical Populations of Conopomorpha sinensis Bradley |
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| DOI：10.16768/j.issn.1004-874X.2024.09.011 |
| 中文关键词: 荔枝蒂蛀虫 地理种群 遗传多样性 遗传分化 简化基因组测序 系统进化树 |
| 英文关键词: Conopomorpha sinensis Bradley geographical populations genetic diversity genetic differentiation simplified genome sequencing phylogenetic tree |
| 基金项目:国家荔枝龙眼产业技术体系专项（CARS-32）；广州市科技计划项目（2024E04J1256） |
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| 中文摘要: |
| 摘 要：【目的】明确荔枝蒂蛀虫（Conopomorpha sinensis Bradley）不同地理种群的遗传结构特征、遗传多
样性水平及分子变异情况，为其起源和演化研究提供科学证据，为不同区域的荔枝蒂蛀虫防治提供参考信息。【方法】
选用我国南方 5 个产区的荔枝蒂蛀虫为材料，利用 GBS（Genotyping-by-sequencing）技术对样本进行基因分型，再
利用 SAMTOOLS 软件鉴定单核苷酸多态性（SNP）位点，根据 SNP 基因分型结果构建系统进化树，并进行主成分
分析。【结果】利用 GBS 技术对 5 个地区荔枝蒂蛀虫进行测序，共获得高质量有效数据 27.39 Gb（平均每个样本 5.48
Gb）、高质量 SNP 位点 11 767 个。系统进化树分析表明，四川泸州种群单独聚为一支，与其他 4 个种群亲缘关系较远。
主成分分析结果显示，5 个地区荔枝蒂蛀虫可划分为 2 个类群，四川泸州种群单独为一簇，明显区分于其他 4 个种群。
主成分分析和系统进化树分析均表明不同地理来源的荔枝蒂蛀虫在遗传水平上存在明显差异。种群遗传多样性分析
结果表明，5 个种群观测杂合度（Ho）为 0.2099~0.2531、期望杂合度（He）为 0.1937~0.2203、核苷酸多样性（π）
为 0.2495~0.2748，表明 5 个荔枝蒂蛀虫种群的遗传多样性水平较低。AMOVA 分析结果表明，荔枝蒂蛀虫种群间的
遗传变异（0.87%）小于种群内（99.13%），表明变异主要来源于种群内而不是种群间。【结论】不同地区的荔枝
蒂蛀虫种群遗传多样性水平较低，其中，四川泸州种群遗传分化程度较高，与其他 4 个种群亲缘关系较远。 |
| 英文摘要: |
| Abstract: 【Objective】The study was conducted to clarify the genetic structure characteristics, genetic diversity level
and molecular variation of different geographical populations of Conopomorpha sinensis Bradely, with an aim to provide
scientific evidence for the study of its origin and evolution and provide reference information for the control of this pest in
different regions.【Method】 Using the C. sinensis from 5 production areas in southern China as materials, using GBS (Genotyping-by-sequencing) technology was used for samples genotyping, then SNP locus was identified with SAMTOOLS
software, and phylogenetic tree was constructed according to SNP genotyping results. Finally, principal component analysis was
performed.【Result】Sequencing of C. sinensis from 5 regions was conducted by GBS technology. A total of 27.39 Gb of highquality effective data were obtained, with an average of 5.48 Gb per sample. A total of 11 767 high-quality single nucleotide
polymorphism (SNPs) loci were obtained. Phylogenetic tree analysis showed that the genetic relationship of Luzhou population
was far from that of other populations. The results of principal component analysis showed that C. sinensis was divided into
2 groups in 5 regions, and the Luzhou population was significantly different from the other 4 populations. Both principal
component analysis and phylogenetic tree analysis revealed that there were obvious differences in genetic level among C.
sinensis from different geographical origins. The results of genetic diversity analysis showed that the observed heterozygosity was
0.2099-0.2531, the expected heterozygosity was 0.1937-0.2203, π was 0.2495-0.2748, indicating that the genetic diversity
level of C. sinensis population was low. The results of AMOVA analysis showed that the genetic variation between populations
(0.87%) was smaller than that within populations (99.13%), indicating that the variation mainly came from within populations
rather than between populations.【Conclusion】The genetic diversity level of C. sinensis in different geographical populations
was low, while the genetic differentiation degree of Luzhou population in Sichuan was high, and its genetic relationship was
distant with other populations. |
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