| 贾文庆，刘会超，郝俊富.金合欢组织培养技术研究[J].广东农业科学,2012,39(2):40-41 |
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金合欢组织培养技术研究 |
| Study on tissue culture of Acacia dealbat |
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| DOI： |
| 中文关键词: 金合欢 无菌苗 不定芽 增殖培养 |
| 英文关键词: Albizzia aseptic seedling adventitious bud multiplication culture |
| 基金项目: |
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| 摘要点击次数: 1701 |
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| 中文摘要: |
| 以金合欢的种子为外植体进行离体培养，初步建立了金合欢离体培养再生体系。结果表明：最适宜诱导愈伤组织和不定芽的培养基是MS+6-BA 1.5 mg/L+NAA 0.1 mg/L；最适宜的增殖培养基是MS+6-BA 2.0 mg/L+NAA 0.2 mg/L；最适宜诱导生根的培养基是1/2MS+IBA 0.4 mg/L。 |
| 英文摘要: |
| The seeds were used as explant to carry out tissue culture of Acacia dealbat, the system of efficient plant regenerations was established initially. The results showed that the explants of Albizzia treated by 0.15% HgCl2 for 10 min could obtained low rate of dead and infection, straight insertion seed is best inoculation method. The optimal culture mediums for callus tissue and adventitious bud induction was MS+6-BA 1.5 mg/L+NAA 0.1 mg/L, the optimal enrichment medium was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L, the optimal rooting medium was 1/2MS+IBA 0.4 mg/L. |
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