| 蒋骄云，冯龙，许世杰，李园园，曲宪成.黄鳝性逆转相关基因的克隆和表达[J].广东农业科学,2012,39(2):106-109 |
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黄鳝性逆转相关基因的克隆和表达 |
| Clone and expression of a novel sex-related gene in gonads during sex reversal in the rice field eel（Monopterus albus） |
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| DOI： |
| 中文关键词: 黄鳝 性腺 差异表达基因 RT-PCR RACE |
| 英文关键词: Monopterus albus gonad differentially expressed gene RT-PCR RACE |
| 基金项目: |
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| 摘要点击次数: 1546 |
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| 中文摘要: |
| 运用cDNA 末端快速扩增（Rapid-Amplification of cDNA Ends,RACE)技术和半定量RT-PCR 技术，克隆了黄鳝（Monopterus albus）性腺差异表达基因（F64）的cDNA全长序列，分析了该基因于各期性腺组织的表达情况。结果表明，F64 基因cDNA全长1 721 bp，含有142 bp 的5'-UTR、268 bp 的3'-UTR、1 311 bp 的开放阅读框（ORF），共编码436 个氨基酸；在线SignalP 分析表明，F64 亚基肽链不存在信号肽，为非经典分泌蛋白；同源性分析结果显示该基因无同源性序列，视为新报道的基因；F64 在卵巢发育早期不表达，从排卵的吁期卵巢开始表达，随着卵巢的败育和精巢的发育，表达量明显升高。 |
| 英文摘要: |
| Using rapid-amplification of cDNA ends and semi-quantitative RT-PCR technique, the full length of the differentially expressed gene (F64) was obtained and the expression in the rice field eel (Monopterus albus) was conducted at different gonad developmental stage. The results showed that the full length of F64 was 1 721 base pair (bp) that comprises of a 5'-UTR of 142 bp, a 3'
-UTR of 268 bp and an open reading frame (ORF) of 1 311 bp which encoded a 436 amino acid with no signal peptide. Homology analysis suggested that it was a new reporter gene. F64 was not transcription at the early stage of ovary and began to express in ovary at V stage, with the ovary abortive and testis development the transcription level was significantly increased. |
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