文章摘要
杜冠魁,周思慧,朱丽娣,李栎,肖曼.动物源性激素DHEA对拟南芥植株根系生长发育的影响[J].广东农业科学,2013,40(3):29-31
动物源性激素DHEA对拟南芥植株根系生长发育的影响
Effect of DHEA on the growth and development of root system of Arabidopsis seedling
  
DOI:
中文关键词: DHEA  葡萄糖-6-磷酸脱氢酶  抑制  氧化状态
英文关键词: DHEA  glucose-6-phosphate dehydrogenase  inhibition  redox state
基金项目:2011 年海南医学院科研培育基金(HY2010-007)
作者单位
杜冠魁,周思慧,朱丽娣,李栎,肖曼 海南医学院生物化学教研室海南医学院理学院袁海南医学院生物学教研室 
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中文摘要:
      用DHEA处理拟南芥种子,观察经DHEA处理后拟南芥幼苗根系的生长状态,并研究其体内氧化还原状态的变化。结果表明:经DHEA 处理后,拟南芥幼苗的主根缩短了20%,葡萄糖-6-磷酸脱氢酶(G6PD)酶活降低,过氧化氢(H2O2)、丙二醛(MDA)及还原性谷胱甘肽(GSH)含量升高,抗氧化酶总超氧化物歧化酶(T-SOD)和过氧化物酶(POD)活力均增强,但过氧化氢酶(CAT)活力下降。说明动物源性激素DHEA在植物中通过抑制G6PD 的酶活,导致植株内的氧化还原状态发生变化,从而影响了主根的生长能力。
英文摘要:
      The Arabidopsis seeds were treated with DHEA to observe the alteration of root elongation and redox state of Arabidopsis seedings. The results showed that the primary root length was 80% of the control and the glucose-6-phosphate dehydrogenase activity decreased; the H2O2, MDA and GSH contents increased; the activity of T-SOD and POD was improved, although CAT activity reduced. These results suggested that the activity of G6PD was inhibited by DHEA, resulted in oxidative stress and inhibition of primary root growth in Arabidopsis seedlings.
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