| 张慧辉，杨雪峰，胡建和，宁红梅，范素辉.紫外分光光度法测定恩诺沙星纳米乳含量方法的建立[J].广东农业科学,2013,40(3):92-94 |
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紫外分光光度法测定恩诺沙星纳米乳含量方法的建立 |
| Determination of enrofloxacin nanoemulsion content by UV spectrophotometry |
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| DOI： |
| 中文关键词: 紫外分光光度计 恩诺沙星 纳米乳 |
| 英文关键词: UV spectrophotometry enrofloxacin nanoemulsion |
| 基金项目:河南科技学院博士科研启动经费(09006) |
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| 中文摘要: |
| 建立紫外分光光度法测定恩诺沙星纳米乳药物含量的方法。恩诺沙星溶液在2.0~7.5 μg/mL检测浓度范围内线性关系良好，标准曲线方程为Y=0.1058X+0.0074(r=0.9997)，最低检测限浓度为0.025 μg/mL；精密度试验的RSD 为1.02%；平均回收率为99.67(±1.70)%，RSD 为1.71%；吸光度值在36 h 内无显著变化；重复性试验的RSD 为0.75%。利用建立的方法，测得恩诺沙星纳米乳中恩诺沙星的含量为115.50 g/L。结果表明，该方法的精密度、回收率、稳定性和重复性均符合要求，可作为恩诺沙星纳米乳中间产品生产过程中的质量控制方法。 |
| 英文摘要: |
| The aim of this paper was to establish an UV spectrophotometry method for the determination of enrofloxacin content in
enrofloxacin nanoemulsion. A good linearity was obtained for enrofloxacin in the range of 2.0~7.5 ug/mL and the standard curvilinear equation was Y =0.1058X+0.0074 (r=0.9997). The lowest detectable limit concentration was 0.025 ug/mL. The relative standard deviation (RSD) of the precision test was 1.02%, the average recovery rate and its RSD was 99.67(依1.70)% and 1.71%,respectively. The absorbance value was no significant change in 36 h and RSD of the repetitiveness test was 0.75%. The mass concentration of enrofloxacin was 115.50 g/L in the preparation of enrofloxacin nanoemulsion. The precision, recovery rate, stability and repetitiveness were all consistent with the request. The method could be used for the quality control of enrofloxacin nanoemulsion. |
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