| 张福莉，刘童童，李创创，杨茂发，杨洪.贵州省五烟区烟草CMV、PVY带毒率检测[J].广东农业科学,2013,40(4):131-133 |
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贵州省五烟区烟草CMV、PVY带毒率检测 |
| The detection on CMV、PVY of tobacco in five different tobacco regions of Guizhou |
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| 基金项目:中国烟草总公司贵州省公司科技项目（201022） |
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| 中文摘要: |
| 采用间接酶联免疫吸附分析法对贵州省5个主要烟草种植区的田间烟草进行了黄瓜花叶病毒病和马铃薯Y病毒病的检测，并采用一步RT-PCR法验证试验的准确性。结果表明：思南、凤冈、德江、正安和清镇5个烟区烟草的CMV带毒率分别为66%、67%、54%、59%和40%，PVY带毒率分别为54%、56%、37%、49%和36%，复合侵染率分别为32%、23%、27%、23%和7%。 |
| 英文摘要: |
| Indirect enzyme linked immunity absorb assay was used to detect Cucumber mosaic virus disease and Potato virus Y of
tobacco in five main tobacco planting areas of Guizhou, and one step RT-PCR method was used to verify the accuracy of the experiment. The results showed that the CMV infectivity of tobacco in Sinan, Fenggang, Dejiang, Zheng'an and Qingzhen were 66%, 67%, 54%, 59% and 40% respectively, the PVY infectivity of tobacco in five tobacco regions were 54%, 56%, 37%, 49% and 36% respectively, and the mixed infection rate were 32%, 23%, 27%, 23% and 7% respectively. |
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