| 于晓惠，朱家红，徐靖，畅文军，张治礼.巴西橡胶HbERF3启动子的克隆与分析[J].广东农业科学,2013,40(5):127-130 |
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巴西橡胶HbERF3启动子的克隆与分析 |
| Cloning and sequence analysis of HbERF3 promoter from Hevea brasiliensis |
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| DOI： |
| 中文关键词: 巴西橡胶 HbERF3启动子 顺式作用元件 乙烯 |
| 英文关键词: Hevea brasiliensis HbERF3 promoter cis-acting elements ethylene |
| 基金项目:国家自然科学基金（31000313）；海南省自然科学基金（309052）；中央级公益性科研院所业务费专项（ITBB110201） |
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| 中文摘要: |
| 根据巴西橡胶HbERF3基因序列，利用GenomeWalking 技术对其启动子进行克隆和分析，得到了长度为1572 bp的HbERF3 基因的5’端上游启动子区，且分析结果表明，该序列具有典型的真核生物核心启动子区和转录起始位点，除了含有多个TATA-box、CAAT-box 等基本顺式元件外，该序列还包含多种与激素应答、逆境胁迫相关的元件。对HbERF3启动子区的克隆和序列分析，为进一步研究HbERF3的表达调控和功能奠定了基础。 |
| 英文摘要: |
| This experiment was designed to study the expression regulation, function in signal transduction and the stress-resistion of HbERF3 gene in Hevea brasiliensis. We designed two specific primers based on the sequence of the ERF3 gene from H. brasiliensis, and a 1572 bp fragment was cloned from the genomic DNA of H. brasiliensis by GenomeWalking method. Sequence analysis showed that the fragment was the 5’upstream promoter region of ERF3 gene and it contained typical eukaryotic core promoters and a transcription start site. The analysis of the cis-acting elements showed that the fragment contained some TATA-box, CAAT-box as well as some elements that responsed to hormone and stresses. The cloning and the sequence analysis of the HbERF3 promoter lay the foundation to a further study of the regulation and function of HbERF3. |
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