文章摘要
李世宗,陈应梅,姚永芳,徐怀亮.果子狸苦味受体Tas2R2基因的克隆与序列分析[J].广东农业科学,2013,40(6):143-147
果子狸苦味受体Tas2R2基因的克隆与序列分析
Cloning and sequence analysis of bitter taste receptor Tas2R2 gene in the Masked palm civet(Paguma larvata0
  
DOI:
中文关键词: 果子狸  苦味受体  Tas2R2基因  克隆  序列分析
英文关键词: Paguma larvata  bitter taste receptor  Tas2R2 gene  cloning  sequence analysis
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作者单位
李世宗,陈应梅,姚永芳,徐怀亮 云南森林自然中心四川农业大学动物科技学院 
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中文摘要:
      采用PCR和克隆测序方法首次从果子狸基因组中获得一全长为1037 bp 的苦味受体Tas2R2 基因DNA 序列。该序列含有完整的1个外显子(无内含子),大小为915 bp,编码304个氨基酸残基。其蛋白质等电点为9.84,分子量为34.87 ku。高级结构预测显示果子狸Tas2R2 蛋白由4个胞外区、7 个跨膜区和4 个胞内区组成,该蛋白上含有N糖基化位点、丝氨酸磷酸化位点、色氨酸磷酸化位点各3个和酪氨酸磷酸化位点1个。亲水性/疏水性分析表明,果子狸Tas2R2 蛋白质为疏水性蛋白,其亲水性区段所占比例较小。种间同源性比较显示,果子狸Tas2R2 基因与猫、犬、大熊猫、牛、马、黑猩猩和小鼠的cDNA 序列同源性分别为93.0%、89.4%、89.9%、85.4%、85.9%、84.6%、72.2%;氨基酸序列同源性分别为88.8%、81.1%、83.2%、75.9%、77.8%、75.5%、61.3%。果子狸、猫、犬、大熊猫、牛、马、黑猩猩和小鼠的Tas2R2 基因编码序列构建的进化树表明果子狸与猫的亲缘关系最近。
英文摘要:
      In this study, an approximately 1 037 bp DNA sequence of bitter taste receptor Tas2R2 gene was obtained by PCR and cloning technique from genomic DNA sample of Masked palm civet (Paguma larvata), which contained complete single exon (without intron), 915 bp in size, encoding 304 amino acid residues. The pI of the protein is 9.84 and its molecular weight is 34.87 ku. High structure prediction shows this Tas2R2 protein comprises 7 transmembrane regions,4 extracellular regions and 4 intracellular regions, and contains three N-glycosylation sites, three serine phosphorylation sites,three tryptophan phosphorylation sites and one tyrosine site. The Tas2R2 is a hydrophobic protein with less hydrophilic components. The homology analysis of the Tas2R2 gene sequences by Clustal W indicated that the cDNA sequence homologies of Tas2R2 gene of Masked palm civet with cat, dog, giant panda, cow, horse, chimpanzee and mouse is 93.0%、89.4%、89.9%、85.4%、85.9%、84.6%、72.2%, respectively and the homologies of corresponding amino acid sequence is 88.8%、81.1%、83.2%、75.9%、77.8%、75.5%、61.3%, respectively. In addition, phylogenetic tree of Tas2R2 gene from these eight species shows that Masked palm civet is the most close to cat.
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