| 赵方方，谢德芳，吕岱竹，林靖凌.高效液相色谱测定香蕉及土壤中戊唑醇残留量[J].广东农业科学,2013,40(9):90-92 |
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高效液相色谱测定香蕉及土壤中戊唑醇残留量 |
| Determination of residue tebuconazole in banana and soil by HPLC |
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| DOI： |
| 中文关键词: 高效液相色谱 香蕉 土壤 戊唑醇 |
| 英文关键词: HPLC banana soil tebuconazole |
| 基金项目:/ |
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| 中文摘要: |
| 建立了戊唑醇在香蕉和土壤中残留量的液相色谱分析方法。试样经乙腈提取，Strata/NH2 固相萃取柱净化后，采用高效液相色谱紫外检测器进行测定，该法最小检出量为2×10-10g，检出限为0.025mg/kg。在添加量为0.025~0.2 mg/kg范围内，戊唑醇在香蕉全蕉中平均回收率为97%~99%，相对标准偏差为2.6%~5.3%；在蕉肉中平均回收率为82%~98%，相对标准偏差为2.0%~4.0%；在土壤中平均回收率为82%~84%，相对标准偏差为1.3%~3.0%。该法准确度高，灵敏度高，线性好，符合农药残留分析的要求，可用于香蕉和土壤中戊唑醇残留量的测定。 |
| 英文摘要: |
| The analysis method for tebuconazole in banana and soil by HPLC (High performance liquid phase chromatography) was established. The sample was extracted from banana and soil with acetonitrile and extrac was purified on Strata/NH2 SPE column, then the sample was injected to the HPLC-UV (High performance liquid phase chromatography-Ultra-violet detector) for analysis. The lowest detection limit of instrument for tebuconazole was 2伊10-10 g, and the limit of quantitation was 0.025 mg/kg. Values of RSD袁S found in the whole fruit were in the range of 2.6%~5.3%, and values of recovery were in the ranges of 97%~99% with 0.025~0.2 mg/kg additive amount. Similarly, values of RSD袁S found in flesh were in the range of 2.0%~4.0%, and values of recovery were in the ranges of 82%~ 98%; values of RSD袁S found in soil were in the range of 1.3%~3.0%, and values of recovery were in the ranges of 82%~84%. The method met the requirement of pesticide residue analysis and could be used to detect the tebuconazole in banana and soil, which showed high accuracy, high sensitivity and good linearity. |
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