| 刘欣欣，徐立新，袁潜华.海南山栏稻HKT2基因片段的克隆与序列生物信息学分析[J].广东农业科学,2013,40(9):128-132 |
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海南山栏稻HKT2基因片段的克隆与序列生物信息学分析 |
| Cloning and sequence biological analysis of HKT2 gene from Shanlan upland rice in Hainan |
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| DOI： |
| 中文关键词: 山栏稻 OsHKT2基因 克隆 生物信息学分析 |
| 英文关键词: Shanlan upland rice OsHKT2 gene cloning sequence biological analysis |
| 基金项目:国家转基因专项-转基因水稻环境安全评价技术（2011ZX08011-001） |
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| 中文摘要: |
| 山栏稻是旱稻的一大类，具有极其重要的社会、生态和经济价值。为研究海南山栏稻运输Na+和K+的分子机制，根据GenBank中已登录的水稻OsHKT2基因的保守序列设计1对引物，从山栏稻幼根中提取总RNA，然后采用PR-PCR(RT-PCR)方法扩增出HKT2基因片段，将扩增出的片段回收后连接到pMd18-T载体上，挑选阳性克隆进行PCR检测后测序，得到一段长为1593 bp的序列。序列分析表明，该片段编码530个氨基酸，与GenBank中注册的水稻OsHKT2基因核苷酸序列的同源性为94%，与OsHKT2蛋白的氨基酸序列同源性为91%。 |
| 英文摘要: |
| Shanlan upland rice is one of major types of upland rice, which is of important social, ecosystem and economic value. Inorder to investigate Na+ and K+ transport mechanism of HKT2 in Shanlan upland rice in Hainan,a pair of primers were designed based onthe conserved sequences of the HKT2 genes registered in GenBank from Oryza sativa. Reverse transcription was performed immediately after the extraction of the total RNA from the root, after the amplification of the partial cDNA by PCR. The PCR product was sub-cloned into pMD18-T vector, used PCR detection the sequence. The sequencing results indicated that the length of the amplified cDNA fragment was 1 593 bp and encoding 530 amino acid. Sequence analysis suggested that the nucleotide sequence and the translated amino acid sequence shared over 94豫and 91豫of homology to HKT2 gene sequences from Oryza sativa, respectively. |
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