文章摘要
林丛发,潘祥华,翁琳琳,卓敏,钟爱清.宁薯10号茎尖离体培养及种薯生产[J].广东农业科学,2013,40(10):14-16
宁薯10号茎尖离体培养及种薯生产
In vitro micropropagatiing of apical meristem and seed breeding to the 10th Ningde-sweet potato
  
DOI:
中文关键词: 宁薯10号  茎尖  脱毒  种薯
英文关键词: 10th Ningde-sweet potato  apical meristem  virus-free曰seed
基金项目:福建省科技重大专项(2008NZ0001)
作者单位
林丛发,潘祥华,翁琳琳,卓敏,钟爱清 宁德市农业科学研究所 
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中文摘要:
      为了保持宁薯10号甘薯品种的优良种性,延长种薯的田间应用年限,运用茎尖分生组织培养,指示植物检测方法获得脱毒苗。结果表明,以MS+6-BA 1.0 mg/L+NAA0.1 mg/L为宁薯10号茎尖初代培养基,成苗率达94.4%;以6-BA0.2 mg/L+NAA0.05 mg/L为茎切段继代增殖培养基,繁殖系数达2.54倍;宁薯10号脱毒苗产量比对照原品种提高16.5%,达到极显著水平,可大面积推广应用。
英文摘要:
      To keep the good character of 10th Ningde-sweet potato, extend the seed application experience in field, virus-free plantlets was obtained by apical meristem culture and indicator plant detection method. The experimental results showed that the first culture medium for apical meristem growth of the 10th Ningde-sweet potato was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L, seedling rate up to 94.4%, and subculture medium for stem segments propagating was 6-BA 0.2 mg/L+NAA 0.05 mg/L, the propagation coefficient was up to 5 times. The yield of its virus-free seedling increased by 16.5% than that of the control original varieties, reached extremely significant level, so it could be popularized in large area.
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