| 雷亮，陶朕，徐凡，吴媚.芹菜叶面微生物吡哆醛还原酶的研究[J].广东农业科学,2013,40(11):25-28 |
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芹菜叶面微生物吡哆醛还原酶的研究 |
| Study on Pyridoxal reductase of phyllosphere microbe from celery leaf |
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| DOI： |
| 中文关键词: 芹菜 叶际微生物 吡哆醛还原酶 酶性质 |
| 英文关键词: celery phyllosphere microbial Pyridoxal reductase enzyme properties |
| 基金项目:/ |
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| 摘要点击次数: 1161 |
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| 中文摘要: |
| 植物叶际有着丰富的生物多样性,是一个复杂的微生态系统,叶际表面微生物通过与宿主植物的互作,改善其栖居环境并促进植物生长。从芹菜叶际生态系统中分离得到吡哆醛还原酶（Pyridoxal Reductase，PLR），采用苯肼衍生方法检测其活性并确定PLR 的来源，对叶际表面微生物进行分离、计数，确定储藏过程中芹菜叶片表面微生物的变化情况，并对PLR的基本酶学性质进行初步研究。结果表明，PLR主要来源于真菌，在相同菌浓度下，PLR活性的强弱依次为真菌跃细菌跃放线菌。该酶的最适温度为37℃，最适反应pH值为7.5，并且在NADPH的参与下，其催化活性显著提高。 |
| 英文摘要: |
| The phyllosphere is a complicated microecological system where colonized by a variety of Microorganism. The epiphytes could improve the dwelling environment and promote the growth of plant through interaction with the host plant. The Pyridoxal Reductase (PLR)isolated from ecosystem of celery leaf, and determine the sources of the PLR with the phenylhydrazine derivative method.Separation and counts of the microorganism separated from celery leaf, determine the changes of microorgenism at the different storage condition, and study on the enzymatic properties of PLR. The results showed that PLR was mainly derived from the fungus of celery leaf surface. The intensity of the PLR activity was fungi>bacteria> actinomycetes at the same bacteria concentration. The enzyme had an optimal temperature and pH at 37益and 7.5. PLR showed a higher catalytic activity of reduction of PL with NADPH. |
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