| 龚兵；张穗强；林悦霞；王秀芳；丁唯嘉；李春远.一株苦槛蓝内生真菌Trichoderma sp.09抗植物病菌产物的研究[J].广东农业科学,2013,40(11):62-65 |
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一株苦槛蓝内生真菌Trichoderma sp.09抗植物病菌产物的研究 |
| Study on metabolites with antifungal activity against plant pathogens of an endophytic fungus Trichoderma sp.09from Myoporum bontioides A. Gray |
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| DOI： |
| 中文关键词: 苦槛蓝 内生真菌 代谢产物 抗真菌 |
| 英文关键词: Myoporum bontioides A. Gray endophytic fungus metabolin antifungal activity |
| 基金项目:国家自然科学基金(21102049);广东省自然科学基金(9451064201003751);广东省教育厅高等学校高层次人才项目(2011);广州市科技计划项目(11C12100771);华南农业大学"211工程"三期重点建设项目（2009B010100001）；华南农业大学科技创新实验项目（SCX12097） |
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| 中文摘要: |
| 从一株苦槛蓝内生真菌Trichoderma sp.09中寻找有抗植物病原菌活性的化合物，利用硅胶柱层析分离到6个化合物，用波谱技术分别鉴定为N-2'-羟基-3'E-十八碳烯酰基-1-O-茁-D-吡喃葡萄糖基-9-甲基-4E,8E-sphingadiene(1)，(4E, 8E)-1-O-(茁-D-吡喃葡萄糖)基-2-(2'-羟基-(E)-3'-十七碳烯酰胺)-3-羟基-9-甲基-4,8-十九碳二烯(2),麦角甾-7,24(28)-二烯-3茁-醇(3),胆甾-4-烯-3-醇(4),正癸酸甲酯(5),正十六碳酸甲酯(6)。化合物1、2为首次从Trichoderma sp.中分离得到，生测结果表明，在0.5μmol/mL 浓度下化合物1对小麦赤霉、香蕉炭疽和柑橘青霉有中度抗菌活性，化合物2对小麦赤霉、香蕉炭疽有中度抗菌活性，对柑橘青霉具有轻度抗菌活性。 |
| 英文摘要: |
| Six compounds were isolated from the fermention liquid of an endophytic fungus Trichoderma sp. 09 from Myoporum bontioides A. Gray by silica gel column chromatography. Their structures were determined to be N-2爷-hydroxy-3'E-octadecenoyl-1-O-茁-D-glucopyranosyl-9-methyl-4E,8E-sphingadiene(1),(4E,8E)-1-O-( 茁-D-glucopyranosyl)-2-( 2爷-hydroxyl-(E)-3爷-heptadecenoylamideo)-3-hydroxyl-9-methyl-4,8-nonadecadiene (2), Ergosta-7, 24 (28)-diene-3茁-ol (3), Cholest-4-ene-3-ol (4), Methyl decanoate (5), Methyl hexadecanoate (6) on the basis of comprehensive spectroscopic analysis. Compound 1 and 2 were isolated fromTrichoderma sp. for the firsttime. Primary bioassay showed compound 1 exhibit moderate inhibtory activity to Fusarium graminearum, Calletotrichum musae and Penicillium italicum袁and compound 2 exhibit moderate inhibtory activity to Fusarium graminearum and Calletotrichum musae, and low inhibtory activity to Penicillium italicum at the concentration of 0.5 μmol/mL. |
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