| 梁彩红,杜宝贵,陈一新,张志胜.火鹤四倍体的诱导与鉴定[J].广东农业科学,2013,40(12):43-45 |
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火鹤四倍体的诱导与鉴定 |
| Tetraploid induction and identification of Anthurium scherzerinum |
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| DOI： |
| 中文关键词: 火鹤 四倍体 愈伤组织诱导 染色体鉴定 |
| 英文关键词: Anthurium scherzerinum tetraploid callus induction chromosome identification |
| 基金项目:/ |
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| 摘要点击次数: 1136 |
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| 中文摘要: |
| 以火鹤二倍体品种‘Smaragd’和‘Artus’的愈伤组织为诱导材料，利用秋水仙素处理的方法进行火鹤四倍体的离体诱导。结果发现，火鹤四倍体诱导率因基因型、秋水仙素处理浓度及处理时间不同而异。不同品种的四倍体诱导率不同，低浓度的秋水仙素的诱导率要高于高浓度的诱导率：相同浓度处理下，长时间处理的诱导率比短时间处理的诱导率要高。两个品种分别在含0.2 g/L秋水仙素浓度的液体培养基中振荡培养14d的四倍体诱导率最高，分别为52.50%和61.90%。随着秋水仙素浓度的增加，愈伤组织的绝对生长速度和平均芽分化数逐渐降低。改良压片法是鉴定火鹤四倍体的可靠方法。 |
| 英文摘要: |
| Calli of two diploid varieties of 'Smaragd'and ‘Artus’, were employed to the tetraploid induction of Anthurium scherzerinum by colchicine treatment method. The results showed that the induction rate depengded on the genotype of A. scherzerinum,the concentrations of colchicene treatment and duration of treatment. The induction rate was different between different genotypes. The lower the colchicine concentration was, the higher the induction rate was. At the same concentration, the longer the duration of treatment was, the higher the induction rate was. The highest induction rate of the two genotypes was 52.5% and 61.90%, separately, which were cultured on the liquid medium contained 0.2 g/L colchicine for 14 d on a rotator. The higher the concentration of colchicene treatment was, the lower the absolute growth rate and the average amout of bud differenation were. The improved squash method was the accurately method of the tetraploid identification of A. scherzerinum. |
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