| 谢宗升，童桂香，廖荣秋，黎小正，廖永志，陈静，吴伟军，韦信贤.斑点叉尾鮰柱形病病原的分离鉴定及药敏试验[J].广东农业科学,2013,40(13):123-126 |
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斑点叉尾鮰柱形病病原的分离鉴定及药敏试验 |
| Isolation, identification and sensitivity to antimicrobial agents of columnaris disease pathogen from channel catfish (Ictalurus punctatus) |
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| DOI： |
| 中文关键词: 斑点叉尾鮰 柱形病 柱状黄杆菌 分离鉴定 药物敏感性 |
| 英文关键词: channel catfish columnaris disease Flavobacterium columnare isolation and identification sensitivity to drug |
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| 中文摘要: |
| 从网箱患柱形病的斑点叉尾鮰病灶中分离到一株细菌（YZ130310），经回归感染试验证实其具有高度致死性。经形态学观察、生理生化特性和16S rRNA基因序列分析鉴定，其主要特征为：革兰氏阴性，菌体细长、两端钝圆尧弯曲或直的杆状，菌落浅黄色，边缘不整齐呈根须状；氧化酶、过氧化氢酶阳性，分解酪素和明胶，不分解纤维素、几丁质、酪氨酸、七叶灵和淀粉，硝酸盐还原阳性，吲哚和葡萄糖产气阴性。菌株的16S rRNA基因序列分析结果表明，菌株YZ130310与GenBank上登录的柱状黄杆菌（Flavobacterium Columnare）ATCC 49512株的16S rRNA序列同源性最高，其相似性达99.6%；在基于16S rRNA基因序列构建的系统发育树中，菌株YZ130310与柱状黄杆菌（AY095342）聚为一支。综合形态及生理生化特点和分子生物学的分类鉴定结果，菌株
YZ130310可鉴定为柱状黄杆菌（F. Columnare）。16种抗菌药物的敏感性试验结果表明，菌株YZ130310对头孢哌酮等7种药物敏感，对阿米卡星和氧氟沙星2种药物中度敏感，对头孢氨苄等7种药物存在不同程度的耐药性。 |
| 英文摘要: |
| A strain bacterium (YZ130310) was isolated from the rotted skin of the channel catfish with columnaris disease. Artificial infection test showed that YZ130310 could lead to 100%mortality of fish in the experimental group with the same signs as the natural infected fish and was the causative pathogen associated with the disease. The bacterial colony was yellow, irregular shape with undulated margin in Shieh solid medium. High-power optical microscope observation revealed that YZ130310 was gram negative and exhibits rodshaped morphology. Physiological and biochemical analysis showed that it had the characteristics of the oxidase, catalase and nitrate reduction positive; hydrolyzation of casein and gelatin; non -hydrolyzation of cellulos, chitin, tyrosine, aesculin and starch; non -fermentation of indole and glucose. The analysis on 16S rRNA gene sequence displayed that YZ130310 had the highest homology in the 16S rRNA nucleotide level with Flavobacterium columnare strain ATCC 49512 with the value 99.6%. Phylogenetic analysis was constructed based on the 16S rRNA gene of F. columnare in the GenBank database and revealed that the strain YZ130310 clustered with F.columnare (AY095342). According to the morphological features, physiological and biochemical characteristics and 16S rRNA gene homology comparison of the bacteria, YZ130310 strain was identified as F. columnare. The sensitivity test using 16 antimicrobial agents showed that strain YZ130310 was sensitive to 7 agents including cefoperazone, was intermediately sensitive to amikacin and ofloxacin, was resistant to 7 agents including cefalexin. |
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