文章摘要
黄明媛,顾文杰,张发宝,卢钰升,解开治,蒋瑞萍.节杆菌YB6发酵滤液稳定性测定及其对盆栽番茄青枯病的防治效果[J].广东农业科学,2013,40(18):75-78
节杆菌YB6发酵滤液稳定性测定及其对盆栽番茄青枯病的防治效果
Stability test of ferment filtrate by Arthrobacter YB6 strain and its control effect on Ralstonia solanacearum
  
DOI:
中文关键词: 节杆菌  番茄青枯病  发酵滤液  抑菌活性  防治效果
英文关键词: Arthrobacter  Ralstonia solanacearum  ferment filtrate  antibacterial activity  control effect
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作者单位
黄明媛,顾文杰,张发宝,卢钰升,解开治,蒋瑞萍 广东省农科院农业资源与环境研究所/农业部南方植物营养与肥料重点实验室/广东省养分资源循环利用与耕地保育重点实验室, 广东广州510640 
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中文摘要:
      节杆菌YB6 (Arthrobacter spp.)为番茄青枯病拮抗菌株,其发酵滤液稳定性测定结果表明,该发酵滤液对热、酸碱及蛋白酶K敏感,在60益及其以上温度失去抑菌活性;强酸强碱条件和蛋白酶K浓度1.5 mg/mL以上均会使其活性显著下降;对光照和紫外线稳定,5 h紫外线照射和35 000 lx光照条件下抑菌活性无显著变化。抗菌谱研究显示,对测试的8种病原菌均有抑制效果,其中对甘蔗黑粉病和小麦赤霉病抑制效果较强,抑菌率分别达88.6%和80.2%。以蘸根对番茄青枯病的防效最好,其防治率达到65.0%。
英文摘要:
      Arthrobacter YB6 strain is an antagonistic bacterium against Ralstonia solanacearum. In the stability test, the ferment filtrat was sensitive to heat, acid-alkali and protease K, it had no antibacterial activity when the temperature was above 60℃, the antibacterial activity would decrease significantly under the condition of strong acid-alkali or the concentration of protease K over 1.5 mg/mL. However,the ferment filtrat had strong stability against light and UV. The antibacterial activity was still stable under the condition of UV irradiation for 5 h or 35 000 L light. The ferment filtrat had wide antibacterial spectrum, and had inhibiting effect against 8 pathogen in the test. It had the best inhibiting effect against Ustilago scitaminea Syd. and Fusarium graminearum, the bacteriostatic rate achieved 88.6% and 80.2%,respectively. The inhibition activity against R. solanacearum by the dipping inoculum method was the best in pot experiments and its biocontrol rate reached 65.0%.
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