| 黄明媛，顾文杰，张发宝，卢钰升，解开治，蒋瑞萍.节杆菌YB6发酵滤液稳定性测定及其对盆栽番茄青枯病的防治效果[J].广东农业科学,2013,40(18):75-78 |
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节杆菌YB6发酵滤液稳定性测定及其对盆栽番茄青枯病的防治效果 |
| Stability test of ferment filtrate by Arthrobacter YB6 strain and its control effect on Ralstonia solanacearum |
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| DOI： |
| 中文关键词: 节杆菌 番茄青枯病 发酵滤液 抑菌活性 防治效果 |
| 英文关键词: Arthrobacter Ralstonia solanacearum ferment filtrate antibacterial activity control effect |
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| 中文摘要: |
| 节杆菌YB6 (Arthrobacter spp.)为番茄青枯病拮抗菌株，其发酵滤液稳定性测定结果表明，该发酵滤液对热、酸碱及蛋白酶K敏感，在60益及其以上温度失去抑菌活性；强酸强碱条件和蛋白酶K浓度1.5 mg/mL以上均会使其活性显著下降；对光照和紫外线稳定，5 h紫外线照射和35 000 lx光照条件下抑菌活性无显著变化。抗菌谱研究显示，对测试的8种病原菌均有抑制效果，其中对甘蔗黑粉病和小麦赤霉病抑制效果较强，抑菌率分别达88.6%和80.2%。以蘸根对番茄青枯病的防效最好，其防治率达到65.0%。 |
| 英文摘要: |
| Arthrobacter YB6 strain is an antagonistic bacterium against Ralstonia solanacearum. In the stability test, the ferment filtrat was sensitive to heat, acid-alkali and protease K, it had no antibacterial activity when the temperature was above 60℃, the antibacterial activity would decrease significantly under the condition of strong acid-alkali or the concentration of protease K over 1.5 mg/mL. However,the ferment filtrat had strong stability against light and UV. The antibacterial activity was still stable under the condition of UV irradiation for 5 h or 35 000 L light. The ferment filtrat had wide antibacterial spectrum, and had inhibiting effect against 8 pathogen in the test. It had the best inhibiting effect against Ustilago scitaminea Syd. and Fusarium graminearum, the bacteriostatic rate achieved 88.6% and 80.2%,respectively. The inhibition activity against R. solanacearum by the dipping inoculum method was the best in pot experiments and its biocontrol rate reached 65.0%. |
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