文章摘要
曾鑫,常平安,李月华,曲绮雯,杨雪娇,黄飞飞.鸡GDE4编码基因的克隆与分析[J].广东农业科学,2013,40(24):128-131
鸡GDE4编码基因的克隆与分析
Cloning and analysis of encoding sequence of chicken glycerophosphodiester phosphodiesterase 4
  
DOI:
中文关键词: 甘油磷酸二酯酶4    编码序列  结构
英文关键词: glycerophosphodiester phosphodiesterase 4  chicken  encoding sequence  structure
基金项目:国家自然科学基金(31271523);重庆市自然科学基金(cstc2013jcyjA10005)
作者单位
曾鑫,常平安,李月华,曲绮雯,杨雪娇,黄飞飞 重庆邮电大学生物信息学院 
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中文摘要:
      为了揭示鸡甘油磷酸二酯磷酸二酯酶4(GDE4)的结构和功能,采用逆转录PCR的方法克隆了其编码cDNA序列,并对其进行了详尽的生物信息学分析。结果表明,鸡GDE4 编码cDNA含有939个核苷酸袁编码312个氨基酸;鸡GDE4具有典型甘油磷酸二酯酶蛋白结构域,是一种跨膜蛋白;GDE4是一种进化保守蛋白,在物种间具有较高的一致性;鸡GDE4的三维结构与磷酸丙糖异构酶桶状结构域结构相似,并且其催化位点位于其中。
英文摘要:
      In order to reveal the structure and function of chicken glycerophosphodiester phosphodiesterase 4 (GDE4),the coding cDNA sequence of chicken GDE4 was cloned by reverse transcription PCR and analyzed by bioinformatical tools in detail. The results showed that coding cDNA of chicken GDE4 contained 939 nucleotides encoding 312 amino acids. Chicken GDE4 was a transmembrane protein with a classical glycerophosphodiester phosphodiesterase domain, was an evolutionarily conserved protein and had a high similarity anmong sepcies. The 3-D model of chicken GDE4 resembled a triose-phosphate-isomerase (TIM) barrel domain and its catalytic active sites were located in.
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