| 李林轩1袁2袁林伟1袁李翠1袁韦丽勤3袁李荣峰3袁韦坤华1.半枝莲组织培养技术研究[J].广东农业科学,2014,41(2):38-40 |
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半枝莲组织培养技术研究 |
| Study on tissue culture of Scutellaria barbata D. Don |
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| DOI： |
| 中文关键词: 半枝莲 继代培养 生根培养 分裂素 生长素 |
| 英文关键词: Scutellaria barbata successive transfer culture rooting culture cytokinin growthhormone |
| 基金项目:国家星火计划项目2011GA790002 |
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| 摘要点击次数: 1444 |
| 全文下载次数: 531 |
| 中文摘要: |
| 药用植物半枝莲是一种应用范围广使用量大和药效功能多的传统中药,近年研究表明其还具有良好
的抗肿瘤作用,研究了不同激素及添加量对半枝莲继代培养和生根培养的影响,结果表明院不同的细胞分裂素和生
长素比例对半枝莲继代培养的影响不同半枝莲继代培养最适培养基为MS+6-BA 1.0 mg/L+NAA 0.2 mg/L+IAA 0.2
mg/L,不同的细胞分裂素和生长素比例对半枝莲根的生长影响也不同,半枝莲壮苗生根最适培养基为1/2MS+ NAA
1.0 mg/L+IBA 0.2 mg/L。 |
| 英文摘要: |
| Scutellaria barbata is a medicinal plant with wide range of application, large amount of use and variety of
efficacy, recent studies indicate that it also has good anti-tumor effect. In this research, the effects of different kinds and
quality of hormone on the subculture and rooting culture of S. barbata were studied. The results showed that the influences of
different cytokinin and auxin ratio on subculture and rooting culture of S. barbata were different, the best subculture medium
was MS+6-BA 1.0 mg/L+NAA 0.2 mg/L+IBA 0.2 mg/L, the best rooting medium was 1/2MS+ NAA 1.0 mg/L+IBA 0.2 mg/L. |
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