文章摘要
王玉林1,周薇樱1,钱泓宇1,陈天任1,余素芹1,3,谢国文1,2,胡位荣1,柯德森1,2.特效植物营养素对迟菜心淀粉酶活力 及种子后代的影响[J].广东农业科学,2014,41(3):41-45
查看全文    HTML 特效植物营养素对迟菜心淀粉酶活力 及种子后代的影响
Evaluation of viability and correlation withgermination rate for gardenia seeds
  
DOI:
中文关键词: 特效植物营养素  增城迟菜心  后代  淀粉酶活力与产量  POD 活力
英文关键词: Gardenia seed  germination  viability  correlation
基金项目:国家级大学生创新创业训练计划项目(20121107 8028);广州大学服务经济社会项目(2012008
作者单位
王玉林1,周薇樱1,钱泓宇1,陈天任1,余素芹1,3,谢国文1,2,胡位荣1,柯德森1,2 1.广州大学生命科学学院广东广州5100062.植物抗逆基因功能研究广州市重点实验室 广东广州5100063.贵州师范大学生物工程技术开发研究中心贵州贵阳550001 
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中文摘要:
      利用特效植物营养素(SPNE)分别喷施迟菜心2 次、1 次,用清水喷施作对照,首次探讨喷施次数对迟菜 心当代淀粉酶活力与产量的影响。将喷施SPNE 2次和对照的种子进行了分析,研究了SPNE 的表观遗传效应对后 代幼苗过氧化物酶(POD)活力的影响。结果表明,SPNE 喷施增城迟菜心后,淀粉酶活力显著提高,迟菜心产量大幅 度提高,且随着喷施次数增加,两者对应增加,喷施2 次的比喷施1 次的高,喷施1 次的比对照高,相关分析结果表 明,淀粉酶活力与迟菜心产量呈正相关。SPNE 喷施迟菜心2 次后种子也比对照大幅增产,种子千粒重增加,发芽势 显著增加,种子后代活力增强。幼苗的叶面积更大,根系更发达,孕韵阅活力极显著提高,抗逆性增强,长势更佳。同时, 进一步探讨了SPNE 在迟菜心后代的表观遗传效应产生机理。
英文摘要:
      TTC staining, BTB indicator staining and red ink staining were used respectively to evaluate seed viability of Gardenia jasminoides Ellis from five habitats. Influences of culture bed (vermiculite, gauze, paper, sand) and temperatures (30,25,20,15mtemperature and 30/20, 25/15 variable temperature) on the germination rate were examined, and germination conditions were optimized to investigate the optimal seed viability test methods and the relationship between seed viability and germination rate for Gardenia jasminoides Ellis. The results showed that BTB indicator method is the best one for seed viability determination, and the filter bed is the best medium in seeds germinateon. Gardenia seeds could reach the highest germination rate when they are incubated at 30 益. Since the correlation coefficient is 0.979 for viability determined by BTB indicator and germination rate of gardenia seed, a significant positive correlation was proposed for gardenia seed viability(X) and germination rate(Y), and the regression equation was Y =1.219X-0.199.
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