| 刘红1，曾建勇1，李震源2.硫酸多粘菌素E 在鸡蛋内的残留量
检测及药代动力学研究[J].广东农业科学,2014,41(3):108-111 |
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硫酸多粘菌素E 在鸡蛋内的残留量
检测及药代动力学研究 |
| Study on preparation technology optimization of Auriculariaauricular polysaccharide by response surface method |
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| DOI： |
| 中文关键词: 硫酸多粘菌素E 鸡蛋 药代动力学 高效液相色谱-三重四级杆串联质谱 |
| 英文关键词: Auricularia auricular polysaccharide high pressure homogenization cellulose central combination
testing |
| 基金项目:中山市科技计划项目（20114A192） |
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| 摘要点击次数: 1530 |
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| 中文摘要: |
| 饲养20 只正处于产蛋旺期的母鸡，单笼饲养，以5 mg/kg 体重的单剂量肌注硫酸多粘菌素耘，早晚给
药，2 次/d，连续注射5 d，其余正常饲喂。建立鸡蛋中硫酸多粘菌素E 的高效液相色谱-三重四级杆串联质谱（HPLCMS/
MS）检测方法，从肌注第1 d开始捡蛋并检测鸡蛋内的药物残留，直至药物残留消除。以Winnonlin 药物动力学参
数计算程序处理鸡蛋中药物浓度-时间数据关系，药动学主要参数为院鸡蛋中药物的达峰浓度（Cmax）为1 411.252(依
578.2536) 滋g/kg，达峰时间（Tmax）为6.425844(依0.666114) d，零阶矩曲线下面积（AUC）为10 647.39(依3159.706) 滋g/kg窑d，
吸收半衰期（K01-HL）为2.173256(依1.115728) d，消除半衰期（K10-HL）为2.237842(依1.072385) d，清除率（CL）为
0.000503(依0.000124) L/kg窑d。 |
| 英文摘要: |
| The process of dissolving Auricularia auricular polysaccharide out effectively according to the
characteristics of its leathery cell wall was studied. This experiment used the combination of high pressure homogenization
and enzyme method, and optimized the extraction process of polysaccharides from A. auricula by using central composite
design method with Minitab statistical software. The results showed that, A. auricular was homogenized under
homogenization pressure 8~10 MPa for 10~12 min as broken pretreatment, then 0.05 mol/L sodium citrate buffer solution
was used as solvent to extract for 2 h at 80. Cooling to 45, 355 U/g cellulase was added and the reaction lasted 1.2 h
in the liquid ratio of 148 in pH 5.0. Then we adjusted pH to neutral with NaOH, and quickly raised the temperature to
85, stayed 1 h for enzyme inactivation. Under above conditions, A. auricula polysaccharides yield reached 15.37%. |
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