| 黄久香1，刘宪宽1，叶永昌2，刘颂颂2，莫罗坚2，庄雪影1.东莞与邻近地区土沉香居群的叶形态
和遗传多样性分析[J].广东农业科学,2014,41(3):153-158 |
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东莞与邻近地区土沉香居群的叶形态
和遗传多样性分析 |
| Separation, purification and structure identification of theantimicrobial substances from Antarctic psychrotrophicbacterium Pseudomonas sp. P4-11 |
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| DOI： |
| 中文关键词: 土沉香 ISSR 遗传多样性 遗传结构 |
| 英文关键词: Antarctic bacteria antimicrobial substance separation and purification structure identification nonylphenol ethoxylates |
| 基金项目:东莞市科技计划资金（201010810105）；广东省林
业科技创新专项资金（2009KJCX002-04）；东莞市林业科学研究 |
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| 中文摘要: |
| 利用ISSR 技术分析了土沉香也Aquilaria sinensis（Lour.）Gilg.页4 居群的遗传多样性及遗传分化。应用9 条
引物从4 个居群共221 份样品中共扩增出105 个位点，其中多态性位点为104 个，多态位点百分率（PPB%）为
99.05%。等位基因数（Na）和有效等位基因数（Ne）分别为1.9905 和1.5854；Nei爷s 基因多样性指数（H）为0.3390，
Shannon 信息指数（I）为0.5054，表明土沉香种内存在较丰富的遗传多样性。土沉香4 个居群总的遗传变异（Ht）为
0.3236，居群内的遗传变异（Hs）为0.2500，居群间的遗传分化系数（Gst）为0.2274，基因流（Nm）为1.6983。UPGMA 聚
类分析结果显示，电白居群和大岭山居群优先聚类，然后再与清溪居群聚类，而广西居群与其余3 个居群明显分离，
显示出较大的遗传分化；电白居群的大叶类群的分化大于小叶类群；东莞大岭山小叶类群与电白居群遗传相似性较
高，而大岭山大叶类群与清溪居群的遗传相似性较高。基于该研究结果，认为东莞大岭山居群部分来自清溪自然居
群，部分与电白居群来源相同，它们均来源于海南。 |
| 英文摘要: |
| The antimicrobial substances of Antarctic psychrotrophic bacterium Pseudomonas sp. P4-11 were studied in
this paper. The results of study on temperature and pH stability of bioactive substances of fermented culture showed that it
had better stability at the range of 30~60 and pH 6~10. By bioassay-guided, two antimicrobial substances (T2-20-7-11-5
and T2-20-7-11-7) were isolated by ethyl acetate extraction, silica gel column chromatograph and HPLC. The structure of
T2-20-7-11-5 and T2-20-7-11-7 were both identified as nonylphenol ethoxylates. The effect of T2-20-7-11-5 on the
ultrastructure of Fusarium oxysporum spore was observed by scanning electron microscope (SEM). The compound might
have a major role in the cell wall or cell membrane, causing leakage of cell contents and consequently resulting in spores
death. |
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