| 王哲1，冯春1，晋曦1，马严1，傅松玲1，陈汉2，杜勤智3.不同封育年限下石灰岩山地植被
细根生物量及其动态[J].广东农业科学,2014,41(3):170-174 |
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不同封育年限下石灰岩山地植被
细根生物量及其动态 |
| Research progress of degradome sequencing method for plants |
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| DOI： |
| 中文关键词: 封山育林 石灰岩山地 细根 生物量 |
| 英文关键词: degradome sequencing method miRNA high-throughput sequencing mRNA target |
| 基金项目:国家“十二五”科技计划项目（2011BAD38B0205）；
安徽农业大学稳定与引进人才项目（YJ2011-5） |
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| 中文摘要: |
| 利用土钻法对不同封育年限下石灰岩山地植被细根生物量进行对比研究。结果表明院封育5 年林分细根
生物量为6.6287 t/hm2，封育10 年林分细根生物量为2.3063 t/hm2，封育20 年林分细根生物量为3.1316 t/hm2，封育
30 年林分细根生物量为2.8316 t/hm2；不同封育年限下细根生物量均随着土层深度的增加而降低，0~10 cm 土层的细
根生物量明显高于其他两个土层；不同封育年限细根生物量年动态变化均呈现双峰型。 |
| 英文摘要: |
| miRNA target identification has been one of the study hotspots in plants. Recently, a high-throughput
technique known as degradome sequencing has made it possible to sequence mRNA cleavage products on a large-scale.
Computational methods now exist to use these data to find targets of conserved and newly identified miRNAs. This
technology has been applied to study the regulation modes of miRNAs on their target genes during plant growth,
development and environmental stress responses. In this paper, the protocols, analysis tools and application examples of
degradome sequencing in plants were reviewed to provide useful knowledge background for studying gene regulation
network. |
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