| 张剑亮，王丽，郑锦荣，何琴，曹干，韩福光，李智军.麻疯树种质资源nrDNA ITS 序列的遗传分析[J].广东农业科学,2014,41(4):36-39 |
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麻疯树种质资源nrDNA ITS 序列的遗传分析 |
| Genetic divergence analysis of Jatropha (Jatropha curcus L.)germplasm based on nuclear ribosomal DNA ITS sequence |
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| DOI： |
| 中文关键词: 麻疯树 nrDNA ITS 遗传多样性 遗传基础 |
| 英文关键词: Jatropha curcas L. nrDNA ITS genetic divergence genetic variation |
| 基金项目:科技部国际科技合作项目（2010DFA33060）；广
东省科技计划项目（2010B050300012，2011B050700003） |
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| 中文摘要: |
| 通过PCR 扩增并测序分析了18 份不同地理来源的麻疯树种质资源的nrDNA ITS 序列，为今后麻疯树
优良种源的选择和开发利用提供理论依据。测序结果表明，麻疯树各种质资源的ITS 序列为648~649 bp，G+C 含量
在64.10%~64.71%之间；各资源的遗传距离为0.000~0.069，平均值仅为0.010。进化树分析表明，18 个麻疯树种质资
源基本上聚为1 类，没有得到具有较高支持率的分支，且各资源的遗传差异系数极低。本研究结果再次证实麻疯树
种质资源的遗传基础偏窄，遗传变异小。 |
| 英文摘要: |
| The genetic divergency of Jatropha curcas L. germplasm, collected from different countries or districts, was
investigated by using nuclear ribosomal DNA ITS sequence (nrDNA ITS) to provide useful information for selection and
utilization of superior provenance of J. curcas. The sequencing results showed that the size variation obtained among
sequenced nrDNA ITS regions was narrow and ranged from 648 to 649 bp and G+C content was from 64.10% to 64.71%.
By Kimura-2 parameter analysis, low genetic distance of germplasm was found from 0.000 to 0.069 and the overall mean
genetic distance was 0.010. The phylogram showed that no clade was found. And the difference coefficient in UPGMA tree
was less than 0.005. The result indicated that the germplasm of J. curcas has narrow genetic base and low genetic variation. |
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