| 周文漪，阮燕如，邓春兴，薛丹，章群.长江和闽江长体鳜遗传多样性的
细胞色素b全序列分析[J].广东农业科学,2014,41(4):170-172 |
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长江和闽江长体鳜遗传多样性的
细胞色素b全序列分析 |
| Genetic variation of mtDNA Cytb sequences of Sinipercaroulei in Yangtze River and Minjiang River |
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| DOI： |
| 中文关键词: 长体鳜 细胞色素b 遗传变异 |
| 英文关键词: Siniperca roulei mtDNA cytochrome b genetic variation |
| 基金项目:国家自然科学基金（41071034）；暨南大学科研创
新基金（21611426，21613105） |
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| 中文摘要: |
| 对长江水系中江西都昌和闽江水系中福建建瓯2 个群体23 尾长体鳜细胞色素b 全基因的1 141 bp 序
列进行分析，发现15 个变异位点，其中简约信息位点7 个，共有13 个单倍型，总体单倍型多样度（Hd）和核苷酸多态
度（Pi）分别为0.933（依0.030）和0.00241（依0.030），呈现出高单倍型多样性和低核苷酸多样性的特点。中性检验结果显
示Fu爷s Fs为显著负值，核苷酸不配对分析呈现单峰分布，表明长体鳜在历史上经历过种群扩张事件，推测扩张年代
约为6 万年前，为更新世晚期。在邻接树和简约性网络图中不同地理来源的单倍型交错分布，群体间的Fst 和Nm 值
分别为0.06667 和3.5。AMOVA 分析表明，长体鳜群体内遗传差异（95.17%）大于群体间（4.83%）遗传差异，遗传变异
主要是集中在群体内部，表明都昌和建瓯的长体鳜群体间没有出现明显遗传分化，可作为一个管理保护单位。 |
| 英文摘要: |
| 1 141-bp sequence of mitochondrial DNA cytochrome b gene of 23 individuals from 2 populations of Siniperca
roulei collected in Duchang of Yangtze River and Jianou of Minjiang River was sequenced. 15 variable sites including 7
parsimony informative sites defined 13 haplotypes, the global haplotype diversity (Hd) and nucleotide diversity (Pi) were 0.933渊依
0.030冤and 0.00241渊依0.030冤respectively, indicating a pattern of high haplotype diversity and low nucleotide diversity. The significant
negative values of neutral test of Fu爷s Fs and the unimodal mismatch distribution pattern revealed a historical population
expansion deduced at about 60 000 years ago. With the intertwined haplotypes of various geographic populations in Neighborjoining
and TCS tree, the Fst and Nm between two populations were 0.06667 and 3.5 respectively, 95.17% genetic variation
occurred between populations was much higher than 4.83% genetic variation occurred in populations by AMOVA analysis,
indicating that no obvious genetic differentiation was found and they could be protected as a single management unit. |
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