| 黄皓，梁卫驱，罗华建，胡珊，李艳芳，陈仕丽，徐匆.重组大肠杆菌发酵表达菊酯类
农药降解酶培养基优化[J].广东农业科学,2014,41(5):134-137 |
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重组大肠杆菌发酵表达菊酯类
农药降解酶培养基优化 |
| Optimization of medium constituents for pyrethroid pesticidedegradative enzyme expression by recombinantEscherichia coli in shake flask fermentation |
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| DOI： |
| 中文关键词: 菊酯类农药降解酶 重组大肠杆菌 培养基 优化 |
| 英文关键词: pyrethroid pesticide degradative enzyme recombinant Escherichia coli medium optimization |
| 基金项目:广东省农业领域科技计划重点专项（2009A02010
2004）；广东省科技计划项目（2009B020411001）；广东省农业厅
野三高冶农业专项基金项目（粤农函[2009]252 号）；东莞市科技计
划重点项目（200910810112）；东莞市科技计划项目（2007108101
108 |
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| 中文摘要: |
| 通过单因素及正交试验，研究了碳源、有机氮源、无机氮源对重组大肠杆菌Eschericha.coli BL21(DE3)/
pET-28a-est825 菌体生长及产酶的影响，进而优化了产酶培养基，确定最佳培养基组成为院甘油20 g/L，酵母粉20
g/L，硫酸铵5 g/L，NaCl 5 g/L，Na2HPO4窑12H2O 10.7 g/L，KH2PO4 2.7 g/L，硫酸卡那霉素50 滋g/L，pH 7.0。采用此培养基
发酵所得发酵液酶活力较优化前提高了86%。 |
| 英文摘要: |
| The medium for pyrethroid pesticide degradative enzyme expression by recombinant Escherichia coli was
optimized by investigating the effcets of carbon sources, organic nitrogen sources and inorganic nitrogen sources on cell
growth and expression of degradative enzyme through single facor and orthogonal experiments. The results showed that the
optimal medium composition was glycerol 20 g/L, yeast extract 20 g/L, (NH4)2SO4 5 g/L, NaCl 5 g/L, Na2HPO412H2O 10.7
g/L, KH2PO4 2.7 g/L, kanamycin sulfate 50 g/L, pH 7.0. By using the optimum medium, the activity of degradative enzyme
increasedby about 86% compared with the original medium. |
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