| 李沐1,2，黄建华1，江世贵1，林黑着1，邱丽华1，姜松1，杨其彬1，周发林1.蛋白水平对F2代斑节对虾幼虾生长
及消化酶活力的影响[J].广东农业科学,2014,41(5):173-177 |
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蛋白水平对F2代斑节对虾幼虾生长
及消化酶活力的影响 |
| Effects of dietary protein content on growth performance anddigestive emzyme activities of F2 generation Penaeus monodon |
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| DOI： |
| 中文关键词: 斑节对虾 蛋白效率 生长性能 消化酶活性 |
| 英文关键词: Penaeus monodon protein efficiency growth performance digestive emzyme activities |
| 基金项目:国家现代农业产业技术体系建设专项（CARS-
47）；广东省海洋渔业推广专项（A201201B02，A201201C01）；广
东省科技成果转化项目（2012NL026）；中国水产科学研究院基
本科研业务费（2013A0502） |
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| 中文摘要: |
| 研究了饲料蛋白水平对F2代（针对饲料蛋白水平选育）斑节对虾幼虾生长和消化酶活力的影响。试验设
计7 个饲料蛋白质水平组（34%、36%、38%、40%、42%、44%、46%），对选育F2代斑节对虾幼虾（2.84依0.04 g）进行48 d
养殖。结果表明，F2代斑节对虾幼虾在38%饲料蛋白组的增重率和特定生长率均为最高，且饵料系数最低；在34%饲
料蛋白组蛋白质效率最高，与36%、38%饲料蛋白组无显著差异，但显著高于40%及以上的各饲料蛋白水平组；成活
率表现为低蛋白组（34%~42%）显著高于高蛋白组（44%、46%）。斑节对虾常规营养成分受饲料蛋白水平影响，全虾粗
蛋白含量随着饲料蛋白水平的升高呈现出上升趋势，但肌肉中各组的粗蛋白含量无显著差异；全虾粗脂肪含量随着
饲料蛋白水平的升高呈现下降的趋势。胃蛋白酶表现为34%饲料蛋白组最高，随着蛋白质含量的升高而呈现出先上
升后下降再上升的波动，与肝脏蛋白酶的含量显示为波动互补的趋势；胃中淀粉酶的含量均高于肝脏，但胃淀粉酶
在各组间无显著差异；胃和肝脏脂肪酶随着蛋白含量的升高总体表现为下降的趋势。综合生长数据、常规营养成分
和消化酶活力等指标得出，38%饲料蛋白水平较为适宜选育F2代斑节对虾幼虾生长，针对低蛋白水平饲料的选育是
有效的。 |
| 英文摘要: |
| Effects of seven dietary protein contents (34%, 36%, 38%, 40%, 42%, 44%, 46%) on growth performance
and digestive emzyme activities of domestic F2 generation Penaeus monodon were studied under laboratory condition. P.
monodon (2.84依0.04 g) were fed with the seven diets in triplicate for 48 d. The results showed that shrimp fed with 38%
dietary protein achieved the highest weight growth rate (WG) and special growth rate (SGR) and the lowest FCR. P.
monodon fed with 34% dietary protein achieved the highest protein efficiency ratiol (PER), without statistical differences
with that fed with 36%, 38% dietary protein, significantly higher than shrimp fed with 40% and above dietary protein.
Survival rate of low-protein group (34%~42%) was significantly higher than that of high-protein group (44%, 46%). Dietary
protein content also affected the whole body composition of P. monodon. Body crude protein content increased, while body
crude fat content decreased with dietary protein level
increasing, but there was no statistical difference of
muscle crude protein in each group. Pepsin of shrimp fed
with 34% dietary protein was the highest, with the
increase of protein content, it increased first, then
decreased, and then increased again, showed fluctuation
and complementary trend with liver protease content.
Amylase in stomach was higher than that in
hepatopancreas, but without statistical differences with stomach amylase in each group. Lipase in stomach and hepatopancreas showed a downward trend with protein
content increasing. The growth performance and digestive enzyme activities indicated that the effect of feed with low
protein level on domesticated F2 generation P. monodon growth was favorable, suggesting that dietary protein of 38%
was more appropriate. |
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