| 陈强1,2，马帅2，谭德冠2，孙雪飘2，张家明2.绿藻Heterochlorella luteoviridis SAG211-2a
叶绿体16S rRNA基因研究[J].广东农业科学,2014,41(7):142-145 |
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绿藻Heterochlorella luteoviridis SAG211-2a
叶绿体16S rRNA基因研究 |
| Study on 16S rRNA gene structure in the Chloroplastof Heterochlorella luteoviridis SAG211-2a |
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| DOI： |
| 中文关键词: 16S rRNA 16S rDNA 玉类内含子 双发夹结构 |
| 英文关键词: 16S rRNA 16S rDNA Group玉intron double-haipin structure |
| 基金项目: |
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| 中文摘要: |
| 为了研究绿藻Heterochlorella luteoviridis SAG211-2a 叶绿体16S rRNA 基因中内含子和双发夹结构在
rRNA 成熟过程中是否被切除，以来自德国歌德堡大学藻种库的绿藻藻株SAG 211-2a 为材料，提取其基因组DNA
作为模板，PCR 扩增并测序叶绿体16S rRNA 基因，与同源物种的16S rRNA 基因进行比对；并提取SAG211-2a 的总
RNA，用一管法RT-PCR 法扩增16S rDNA 基因成熟转录子后，克隆至pMD19-T 表达载体上，转化大肠杆菌DH5琢，
提取质粒并测序。结果表明，绿藻SAG211-2a 叶绿体16S rRNA 基因中存在1 个长度为46 bp 的双发夹结构(DHE）
和1 个长度为537 bp 的玉类内含子，内含子在rRNA 成熟过程中被切除，而双发夹结构却保留在rRNA 中，从而验证
了双发夹结构在rRNA 成熟过程中没有被切除。 |
| 英文摘要: |
| In order to study whether the intron and double-hairpin structure in the Chloroplast 16S rRNA gene of
Heterochlorella luteoviridis SAG211-2a were removed during the maturation of rRNA, H. luteoviridis SAG211-2a coming
from the algae collection of Gothenburg University in Germany was used as material. The 16S rRNA gene was amplified by
PCR using the extracted H. luteoviridis SAG211 -2a DNA as template, and was compared with the 16S rRNA from
homologous species. Then, the 16S rRNA gene mature transcript was amplified by one step of RT-PCR method using the
total RNA extracted from H. luteoviridis as template and was inserted into expression vector pMD19 -T, then was
transformed to E. coli DH5. Plasmids were extracted and sequenced. The results showed that the Chloroplast 16S rRNA
gene of H. luteoviridis SAG211-2a had a double-hairpin structure (DHE) of 46 bp and a Group玉intron of 537 bp. The
Group I intron was removed in the rRNA maturation, and the double-hairpin structure was retained in the rRNA. Thus
verified that the double-haipin structure was not removed in the rRNA maturation. |
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