| 池洪树1,2、龚晖2、柯翎2、刘晓东2、林天龙2.刺激隐核虫在褐牙鲆上的传代及其生活史观察[J].广东农业科学,2014,41(10):98-101 |
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刺激隐核虫在褐牙鲆上的传代及其生活史观察 |
| Propagation of Cryptocaryon irritans on Paralichthysolivaceus and observation of its life cycle |
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| DOI： |
| 中文关键词: 刺激隐核虫 褐牙鲆 传代 鱼类免疫 |
| 英文关键词: Cryptocaryon irritans Paralichthys olivaceus propagation fish immunology |
| 基金项目:福建省重大专项（2013NZ0002-5）；农业部野948冶
项目（2011-Z11） |
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| 中文摘要: |
| 为保存从福建省长乐市褐牙鲆上分离的刺激隐核虫、并了解其生物学特性、以褐牙鲆为宿主鱼、在水温
22~24益时、对刺激隐核虫进行传代、详细观察其生活史及各阶段的形态结构、比较不同温度处理的包囊孵化率和孵
出幼虫的感染能力、分别采集感染后2 周和4 周的褐牙鲆血清做阻动试验。目前已成功进行了40 个周期的刺激隐
核虫传代保种、每个周期8~10 d、表明褐牙鲆可作为刺激隐核虫的模式宿主鱼。观察包囊的形态结构、发现少数包囊
中存在疑似滋养体并做旋转运动的圆形原生质体。包囊5益冷藏2 个月后刺激隐核虫的孵化率下降至7.6 %、用
1 000 个5益冷藏后孵化的幼虫感染褐牙鲆、未检出滋养体。免疫血清阻动试验表明、感染后2 周和4 周的褐牙鲆血
清对幼虫的阻动效价分别为1颐320 和1颐1 280、说明体液免疫在抵抗刺激隐核虫的感染中发挥了重要作用。 |
| 英文摘要: |
| In order to conserve a strain Cryptocaryon irritans isolated from Paralichthys olivaceus in ChangLe of
Fujian province, the parasites were propagated in laboratory by using P. olivaceus as host, controlling water temperature
between 22益and 24益. The morphological characteristics in each life cycle period were observed and recorded. The
hatchability of tomonts and infection ability of theronts were compared between two groups, as tomonts in one group were
stored in 5益for two months before hatching in 27益and directly hatched in 27益in the other group. Serum collected from
the fish infected after 2 weeks and 4 weeks was detected on clumping and immobilization to theronts. Results showed that
P. olivaceus could be a model host for C. irritans. Up to 40 consecutive cycles were successively established, 8-10 d in one
cycle. Some trophont-like round protoplasts were observed orbiting in the tomont. The hatchability of tomonts dropt to 7.6
% in the first group. And none trophont could be detected on the fish after exposed to 1 000 theronts in this group. The
immobilization titers of serum antibody to theronts could be 1颐320 and 1颐1 280 in two-week infected fish group and fourweek
infected fish group. This indicated that humeral immunity played an important role against C. irritans infection. |
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