| 宋江平1,2，陈雨2,3，汪文娟4，潘大建2,3，曲延英1，陈全家1，朱小源4，李晨1,2,3
（2012B060400004).源宝占抗稻瘟病遗传分析及基因定位[J].广东农业科学,2014,41(12):1-5 |
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源宝占抗稻瘟病遗传分析及基因定位 |
| Genetic analysis and gene mapping of rice blastresistance in an indica rice Yuanbaozhan |
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| DOI： |
| 中文关键词: 稻瘟病 生理小种 基因定位 群体分离分析法(BSA) 隐性群体分析法(RCA) |
| 英文关键词: rice blast physiological race gene mapping bulked segregation analysis (BSA) recessive-class
approaches(RCA |
| 基金项目:国家公益性行业（农业）科研专项（201003021）；
国家现代农业产业技术体系建设专项（CARS-01-24，粤财教 |
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| 摘要点击次数: 1542 |
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| 中文摘要: |
| 稻瘟病是水稻三大病害之一，严重威胁着粮食安全。解决这一问题最安全、有效的措施是选育、推广抗
病品种。源宝占是一份新育水稻材料，经鉴定，源宝占对来自广东各稻作区的25 个菌株中23 个表现为抗性，抗频达
92%，为广谱抗性材料。以源宝占与粤香占进行杂交，构建F1、F2群体，选用GD00-193a 菌株对亲本及世代群体进行
接种鉴定，结果表明F2代单株抗感分离比符合3颐1，这说明源宝占对菌株GD00-193a 的抗性是由一对显性基因或一
个主效QTL 控制。利用群体分离分析法（BSA）结合隐性群体分析法（RCA）将此基因定位于标记RM136 与RM549 之
间。
[2009]356 号）；广东省科技计划项目 |
| 英文摘要: |
| Rice blast, caused by fungus Magnaporthe grisea, is one of the most serious diseases of rice and has become
a serious threat to food security. Yuanbaozhan is a new rice material. Improving the rice resistance to rice blast through
genetic improvement methods is the most effective and safe way in the present. Yuanbaozhan has an broad -spectrum
resistance to rice blast pathogen, and it has high resistance to 23 strains among 25 blast strains collected from different
regions in Guangdong province. Its resistance frequency is 92%. In this study, we made a combination to establish genetic
population F1, F2 among which population is from the crosses between Yuanbaozhan and Yuexiangzhan. Evaluation was
conducted with Magnaprthe oryzae isolate GD00-193a, the results showed that the resistant and susceptible plants in F2
population fitted the ratio of 3:1, indicating that the resistance of Yuanbaozhan to the rice blast was controlled by a
dominant gene or a QTL locus. With the bulked-extrems and recessive-class approach analysis, the rice blast resistance
gene was mapped at the genome region between RM136 and RM549. |
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