| 许卫华1,2，吴珍芳1,3，余波1，贺晓燕1,3，石俊松3，周荣3，刘德武1，李紫聪1.供体细胞系基因座位特异DNA 甲基化多态性
及其与猪克隆效率的关联分析[J].广东农业科学,2014,41(12):165-170 |
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供体细胞系基因座位特异DNA 甲基化多态性
及其与猪克隆效率的关联分析 |
| Study on locus-specific DNA methylation polymorphismsamong donor cell lines and their associations withporcine somatic cell cloning efficiency |
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| DOI： |
| 中文关键词: 猪 供体细胞 DNA 甲基化 多态性 克隆效率 |
| 英文关键词: porcine donor cell DNA methylation polymorphisms cloning efficiency |
| 基金项目:广东省科技计划项目(2011A020102003）；广东院
士工作站项目(2011A090700016）；福建省教育厅科技项目(JB10
158） |
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| 摘要点击次数: 1722 |
| 全文下载次数: 565 |
| 中文摘要: |
| 利用甲基化限制性酶切试验(Combined bisulfite restriction analysis, COBRA）和亚硫酸盐转换PCR
(Bisulfite sequencing PCR , BSP）后测序的方法，筛选猪体细胞克隆优势供体细胞提供基因座位特异的DNA 甲基化
遗传标记。比较8 个发育重要基因的差异甲基化区域(Differentially methylated regions，DMRs）在不同克隆供体细胞系
中的DNA 甲基化多态性，并与猪克隆成绩进行关联分析。结果表明院除了XIST-DMR2 和H19-DMR3 DNA 甲基化变
异幅度较小(低于10%）外，其他基因的相关座位均存在一定的COBRA 多态性，其中，LINE1-DMR1、POU5F1-DMR1
和NANOG-DMR1 等3 个差异甲基化化区域在克隆效率最高细胞系中处均处于最低程度的DNA 甲基化状态，因此，
可作为筛选猪体细胞克隆优势供体细胞系的潜在的DNA 甲基化遗传标记。 |
| 英文摘要: |
| Bisulfite restriction analysis (COBRA) combined with bisulfite sequencing (BS) were firstly carried out to
screen locus-specific DNA methylation markers in donor cell lines for improving porcine somatic cell cloning efficiency.
DNA methylation polymorphisms of eight differentially methylated regions (DRMs) among six donor cell lines and their
potential associations with porcine cloning achievements were analyzed. Result indicated that all DMRs presented relatively
high degree of DNA methylation polymorphisms, except for XIST -DMR2 and H19 -DMR3 with low extent of DNA
methylation variations below 10%. Moreover, three DMRs including LINE1-DMR1, POU5F1-DMR1, and NANOG-DMR1
presented the most DNA hypomethylated status in the most efficient cell line, and thus could be considered as potential
DNA methylation genetic markers for selecting optimum donor cell lines for porcine somatic cell cloning. |
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