| 周丽霞，肖勇袁，杨耀东.油棕转录组SSR 标记开发研究[J].广东农业科学,2014,41(14):136-138 |
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油棕转录组SSR 标记开发研究 |
| Development of SSR markers in oil palm (Elaeis guineensis) based on information from transcriptome sequencing |
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| DOI： |
| 中文关键词: 油棕 转录组 SSR |
| 英文关键词: oil palm transcriptome sequencing SSR |
| 基金项目:国家自然科学基金（31101179） |
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| 摘要点击次数: 1381 |
| 全文下载次数: 791 |
| 中文摘要: |
| 从NCBI网站下载4072 条油棕EST，结合文献报道的油棕果肉转录组序列信息，通过聚类拼接和处理，得到全长为32637.947 kb的无冗余序列20054条。在这些序列中共检索出4 538 个SSR袁检出率为22.6%遥其中以单核苷酸重复基序为主导类型袁出现频率为51.2%，二核苷酸、三核苷酸重复基序的出现频率分别为20.4%和17.9%。随机挑选了400对SSR引物进行PCR 多态性检测，获得29对PCR多态性引物，并随机选取11引物进行验证，在4种不同来源的油棕样本中表现出多态性。 |
| 英文摘要: |
| In this study, combined with oil palm pulp transcriptome sequence information reported in the literature, a
total of 40 728 EST of oil palm from NCBI (http://www.ncbi.nlm nih.Gov/genbank/) were downloaded and analyzed,
resulting in 20 054 non-redundant sequences with total length of 32 637.947 kb. 4 538 SSRs were discovered, and the
frequency of these SSRs was 22.6%. Among them, single nucleotide type was the dominant type, accounting for 51.2%,
followed by dinucleotide(20.4%) and trinucleotide (17.9%). 400 pairs of SSR primers for PCR polymorphism detection were
randomly selected, and 29 pairs of PCR primers were polymorphic, of which 11 primer pairs showed polymorphism among 4
different oil palm varieties. |
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