| 丛铭，万科，禹辉，陈雪，郭博恺，费长飞，王嫱，李祝.烟草青枯病生防菌黑曲霉发酵条件优化研究[J].广东农业科学,2014,41(15):70-75 |
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烟草青枯病生防菌黑曲霉发酵条件优化研究 |
| Optimization of fermentation condition of an Aspergillusniger ctrain against tobacco Ralstonia solanacearum |
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| DOI： |
| 中文关键词: 青枯菌 黑曲霉 发酵 Plackett- Burman设计 响应面法 |
| 英文关键词: Ralstonia solanacearum Aspergillus niger fermentation Plackett- Burman design response surface methodology |
| 基金项目:贵州省烟草公司毕节市公司科技项目（201106）；贵州省农业攻关科技计划项目（黔科合NY字[2011]3053）；贵州省科学技术基金（黔科合J字[2010]2065号）；贵州大学SRT项目（贵大SRT字[2013]082号） |
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| 中文摘要: |
| 以烟草青枯菌Ralstonia solanacearum 为指示菌袁黑曲霉Aspergillus niger 对其的抑菌圈直径为响应值袁
运用单因素试验尧Plackett-Burman渊PB冤设计尧Box-Behnken渊BBD冤设计及响应面Response Surface Methodology渊RSM冤
分析将分离并鉴定的黑曲霉菌株的液体发酵条件进行了优化遥通过单因素试验确定麦芽糖和麦麸分别为最适碳源
和氮源袁通过Plackett- Burman 设计对培养基中相关影响因素的效应进行评价袁并筛选出有显著正效应的MgSO4及
有显著负效应的CaCl2和吐温80 等3 个因素袁使用最陡爬坡路径逼近最大抑菌圈直径袁最后用BBD 设计及RSM 分
析确定了主要影响因素的最佳条件遥结果显示袁经过优化的培养基组分为麦芽糖25.00 g/L尧MgSO4 0.38 g/L尧K2HPO4
0.10 g/L尧KCl 0.50 g/L尧CaCl2 0.05g/L尧FeSO4 0.20 g/L尧麦麸8.50 g/L尧酵母膏1.50 g/L尧吐温80 0.46 mL/L遥采用滤纸片扩
散法检测优化后的发酵液抗菌活性袁抑菌圈直径为37.49(依0.34) mm袁比未经优化测得的抑菌圈直径24.79(依0.72) mm
增大了51.23%遥 |
| 英文摘要: |
| The condition for liquid fermentation of an Aspergillus niger strain was optimized by using Design-Expert software combined with the Box-Behnken Design (BBD) and Response Surface Methodology (RSM) analysis method, and the Ralstonia solanacearum was used as indicator and inhibition zone Aspergillus niger aganst was used as the response value. The optimum carbon and nitrogen for Aspergillus niger were obtained through single factor experiment, which were maltose and wheat bran, respectively. Through Plackett -Burrman design, some factors of fermentation medium were evaluated and three factors had prominent effect on inhibition zone, which were MgSO4, CaCl2 and Tween 80, respectively. Then steepest ascent was used to approach maximum inhibition zone diameter. The BBD and RSM were used to determine the optimal levels of the main influencing factors, as the composition of fermentation medium was optimized as follow: maltose 25.00 g/L, MgSO4 0.38 g/L, K2HPO4 0.10 g/L, KCl 0.50 g/L, CaCl2 0.05 g/L, FeSO4 0.20 g/L, wheat brain 8.50 g/L,yeast paste 1.50 g/L, Tween 80 0.46 mL/L. Under those optimal conditions, the diameter of inhibitory zone increased by 51.23% from 24.79(依0.72) to 37.49(依0.34) mm. |
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